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Phospho-TrkB (Tyr516) Antibody (PA5-36695) in ICC/IF
Immunofluorescence analysis of Phospho-TrkB (Tyr516) was performed using 70% confluent log phase A549 cells, untreated or treated with 25 ng/mL BDNF for 1 hr. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with anti-Phospho-TrkB (Tyr516) Rabbit Polyclonal Antibo... View More
产品信息
PA5-36695
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic phosphopeptide derived from human Trk B around the phosphorylation site of Tyrosine 516
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.2, with 50% glycerol
内含物
0.02% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2553666
产品详细信息
This antibody detects endogenous protein at a molecular weight of 92 and 145 kDa.
Purity is >95% by SDS-PAGE.
靶标信息
NTRK2 (TRKB) belongs to the neurotrophic factor family of related polypeptides central to the development and maintenance of the mammalian nervous system. NTRK2 is the receptor for brain-derived neurotrophic factor (BDNF). Together, NTRK2 and BDNF regulate both short-term synaptic functions and long-term potentiation of brain synapses. TrkB is involved in the development and maintenance of the nervous system. Moreover, Trk B is a tyrosine kinase gene highly related to Trk A. Trk B expression is confined to tissues within the central and peripheral nervous systems. The brain-derived neurotrophic factor (BDNF) and NT-3, but not NGF, can induce rapid phosphorylation on tyrosine of Trk B gp145, one of the receptors encoded by Trk B, although BDNF elicits a response at least two orders of magnitude greater than NT-3. TrkB is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signaling through TrkB leads to cell differentiation. Mutations in the TrkB gene have been associated with obesity and mood disorders. Alternate transcriptional splice variants encoding different isoforms have been found for the TrkB gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: AI848316; BDNF-tropomyosine receptor kinase B; BDNF/NT-3 growth factors receptor; C030027L06Rik; EC 2.7.10.1; GP145 TrkB; GP145-TrkB; GP145-TrkB/GP95-TrkB; GP145TrkB; GP95 TrkB; GP95-TrkB; hyperphagia; kinase TrkB; neural receptor protein-tyrosine kinase (trkB); Neurotrophic tyrosine kinase receptor type 2; neurotrophic tyrosine kinase, receptor, type 2; neurotrophic tyrosine receptor kinase type 2; NTRK 2; OTTHUMP00000021573; OTTHUMP00000021574; OTTHUMP00000021576; OTTHUMP00000021577; Trk B; Trk-B; TrkB tyrosine kinase; Tropomyosin-related kinase B; tyrosine kinase receptor B
基因别名: GP145-TrkB; GP145-TrkB/GP95-TrkB; NTRK2; RATTRKB1; Tkrb; trk-B; TRKB; TRKB1
UniProt ID: (Human) Q16620, (Mouse) Q3TUF9, (Rat) Q63604
Entrez Gene ID: (Human) 4915, (Mouse) 18212, (Rat) 25054
ATP binding
receptor tyrosine kinase binding
protein homodimerization activity
neurotrophin binding
brain-derived neurotrophic factor binding
brain-derived neurotrophic factor-activated receptor activity
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
neurotrophin receptor activity
protein binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
ephrin receptor binding
transmembrane signal receptor
immunoglobulin superfamily cell adhesion molecule
vasculogenesis
neuron migration
positive regulation of protein phosphorylation
inflammatory response
neuromuscular junction development
aging
learning
long-term memory
circadian rhythm
feeding behavior
positive regulation of cell proliferation
response to light stimulus
positive regulation of gene expression
positive regulation of neuron projection development
response to auditory stimulus
glutamate secretion
positive regulation of phosphatidylinositol 3-kinase signaling
peptidyl-tyrosine phosphorylation
central nervous system neuron development
cerebral cortex development
neuron differentiation
brain-derived neurotrophic factor receptor signaling pathway
positive regulation of peptidyl-serine phosphorylation
calcium-mediated signaling using intracellular calcium source
neurotrophin signaling pathway
mechanoreceptor differentiation
regulation of GTPase activity
positive regulation of MAPK cascade
negative regulation of neuron apoptotic process
retinal rod cell development
protein autophosphorylation
regulation of neurotransmitter secretion
oligodendrocyte differentiation
peripheral nervous system neuron development
positive regulation of axonogenesis
regulation of dendrite development
regulation of protein kinase B signaling
positive regulation of synapse assembly
positive regulation of synaptic transmission, glutamatergic
retina development in camera-type eye
long-term synaptic potentiation
cellular response to amino acid stimulus
cellular response to tumor necrosis factor
cellular response to brain-derived neurotrophic factor stimulus
negative regulation of anoikis
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
multicellular organism development
nervous system development
phosphorylation
regulation of metabolic process
cell differentiation
regulation of MAPK cascade
positive regulation of glucocorticoid receptor signaling pathway
cellular response to nerve growth factor stimulus
