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Phospho-ULK1 (Ser638) Antibody (PA5-117270) in WB
Western blot analysis of Phospho-ULK1 (Ser638) in extracts of 293T cells. Samples were incubated with Phospho-ULK1 (Ser638) Polyclonal antibody (Product # PA5-117270) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. 293T cells were treated by oligomycin (0.5 µM) at 37°C for 30 minutes. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% ... View More
产品信息
PA5-117270
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic phosphorylated peptide around S638 of human ULK1 (NP_0035561)
偶联物
形式
Liquid
浓度
0.7 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2901900
产品详细信息
Positive Samples: 293T
Immunogen sequence: TPSSQ
靶标信息
ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.
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仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: APG1; ATG 1; ATG1; ATG1 autophagy related 1 homolog; Autophagy-related protein 1 homolog; C. elegans; EC:2.7.11.1; FLJ38455; Serine/threonine-protein kinase ULK1; Serine/threonine-protein kinase Unc51.1; ULK 1; UNC 51; Unc-51-like kinase 1
基因别名: ATG1; ATG1A; AU041434; hATG1; KIAA0722; mKIAA0722; ULK1; UNC51; Unc51.1
UniProt ID: (Human) O75385, (Mouse) O70405
Entrez Gene ID: (Human) 8408, (Mouse) 22241
protein serine/threonine kinase activity
protein binding
ATP binding
Rab GTPase binding
protein complex binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein kinase binding
Hsp90 protein binding
non-receptor serine/threonine protein kinase
protein phosphorylation
signal transduction
protein localization
regulation of autophagy
positive regulation of autophagy
macroautophagy
positive regulation of macroautophagy
peptidyl-serine phosphorylation
neuron projection regeneration
neuron projection development
negative regulation of protein complex assembly
cellular response to nutrient levels
response to starvation
protein autophosphorylation
axon extension
autophagosome assembly
autophagy
Ras protein signal transduction
axonogenesis
regulation of gene expression
regulation of cell death
phosphorylation
cerebellar granule cell differentiation
radial glia guided migration of cerebellar granule cell
receptor internalization
negative regulation of collateral sprouting
regulation of neurotrophin TRK receptor signaling pathway
mitophagy in response to mitochondrial depolarization
