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Phospho-USP28 (Ser714) Antibody (PA5-106054) in ICC/IF
Immunocytochemistry analysis of Phospho-USP28 (Ser714) in Hela cells (serum starvation). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-USP28 (Ser714) polyclonal antibody (Product # PA5-106054) 1:200 and mouse anti-beta tubulin antibody (1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat a... View More
产品信息
PA5-106054
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Non-human primate
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human USP28(Accession Q96RU2), corresponding to amino acid residues around phosphorylated Ser714.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
sequential chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2817453
产品详细信息
Antibody detects endogenous levels of USP28 only when phosphorylated at Ser714.
靶标信息
Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Deubiquitinating enzyme 28; Ubiquitin carboxyl-terminal hydrolase 28; ubiquitin carboxyl-terminal hydrolase 28 variant 1; ubiquitin specific protease 28; Ubiquitin thioesterase 28; ubiquitin thiolesterase 28; Ubiquitin-specific-processing protease 28
基因别名: 9830148O20Rik; AU022237; KIAA1515; mKIAA1515; USP28
UniProt ID: (Human) Q96RU2, (Mouse) Q5I043
Entrez Gene ID: (Human) 57646, (Mouse) 235323
DNA damage checkpoint
DNA repair
ubiquitin-dependent protein catabolic process
cellular response to DNA damage stimulus
Ras protein signal transduction
cell proliferation
response to ionizing radiation
protein deubiquitination
regulation of protein stability
cellular response to UV
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
proteolysis
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of proteasomal protein catabolic process
