Invitrogen

Phospho-VAV1 (Tyr160) Polyclonal Antibody

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Phospho-VAV1 (Tyr160) Antibody in Immunoprecipitation (IP)

Phospho-VAV1 (Tyr160) Antibody in Immunoprecipitation (IP)

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Phospho-VAV1 (Tyr160) Antibody (44-482) in IP

Vav1 protein immunoprecipitated from K562 cell extracts was resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/mL Vav1 (pY160) antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the i... View More

Phospho-VAV1 (Tyr160) Antibody in Immunoprecipitation (IP)

Phospho-VAV1 (Tyr160) Antibody in Western Blot (WB)

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Phospho-VAV1 (Tyr160) Antibody in Immunoprecipitation (IP) — Vav1 protein immunoprecipitated from K562 cell extracts was resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/mL Vav1 (pY160) antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphotyrosine containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F (ab')2 anti-rabbit IgG alkaline phosphatase (Product # ALI4405) and signals were detected using the Tropix WesternStar™ method. The data show that only the peptide corresponding to Vav1 (pY160) blocks the antibody signal, thereby demonstrating the specificity of the antibody.

Phospho-VAV1 (Tyr160) Antibody in Western Blot (WB) — Fig. 5 Membrane-bound, but not soluble MIC, preferentially enhances NK cell cytotoxicity and associated signaling pathways. a Cytotoxicity of human NKL cell line cultured with labeled target cells C1R, C1R-sMICA, C1R-mMICA at different E/T ratios for 4 h, as assessed by flow cytometry analyses of 7-ADD for tumor cell death. b Cytotoxicity of primary human NK cells against C1R-mMICA with stimulation conditions of sMICA (100 ng/ml) alone or in presence of B10G5 (5 ug/ml) assessed by lactate dehydrogenase (LDH) release. Immunoblot analysis to evaluate the activation of ( c - e ) Vav1 phosphorylated at Tyr 160 (p-Vav1) and PLCgamma2 phosphorylated at Tyr 1217 (p- PLCgamma2); f , g SLP-76 phosphorylated at Tyr 128 (p-SLP-76); and h - j ERK phosphorylated at Tyr 202/Tyr204 (p-ERK) and JNK phosphorylated at Thr183/Tyr185 (p-JNK) in human NK cells at the indicated condition and timepoints. Phosphorylated proteins were normalized to respective total proteins. Individual phospho-proteins, their respective total proteins, and beta-actin were presented from the same blots. The blots were first probed for phospho-proteins, followed by stripping and re-probing for respective total proteins and subsequently for beta-actin. The numbers represent quantified values after normalization and are represented as relative to NK only control conditions. The blots shown are representative of three independent experiments. The bar graphs show the quantitative data of relative protein levels and are rep

产品信息

44-482

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫沉淀 (IP)

Assay-dependent

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A chemically synthesized phosphopeptide derived from a region of human Vav1 that contains tyrosine 160

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533661

靶标信息

This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Tumor-derived NKG2D ligand sMIC reprograms NK cells to an inflammatory phenotype through CBM signalosome activation.

Communications biology

Dhar P,Basher F,Ji Z,Huang L,Qin S,Wainwright DA,Robinson J,Hagler S,Zhou J,MacKay S,Wu JD

44-482 was used in Western Blot to provide a rationale for co-targeting sMIC to enhance the efficacy of the ongoing NK cell-based cancer immunotherapy.

Thu Jul 22 00:00:00 UTC 2021

Tumor-derived NKG2D ligand sMIC reprograms NK cells to an inflammatory phenotype through CBM signalosome activation.

Communications biology

Dhar P,Basher F,Ji Z,Huang L,Qin S,Wainwright DA,Robinson J,Hagler S,Zhou J,MacKay S,Wu JD

44-482 was used in Western Blot to provide a rationale for co-targeting sMIC to enhance the efficacy of the ongoing NK cell-based cancer immunotherapy.

Thu Jul 22 00:00:00 UTC 2021

IFT20 controls LAT recruitment to the immune synapse and T-cell activation in vivo.

Proceedings of the National Academy of Sciences of the United States of America

Vivar OI,Masi G,Carpier JM,Magalhaes JG,Galgano D,Pazour GJ,Amigorena S,Hivroz C,Baldari CT

44-482 was used in western blot to investigate the control of LAT recruitment to the immune synapse and T-cell activation in vivo by IFT20

Tue Jan 12 00:00:00 UTC 2016

Reduced TCR signaling potential impairs negative selection but does not result in autoimmune disease.

The Journal of experimental medicine

Hwang S,Song KD,Lesourne R,Lee J,Pinkhasov J,Li L,El-Khoury D,Love PE

44-482 was used in western blot to study the influence of TCR signaling to negative selection and regulatory T cell development

Mon Sep 24 00:00:00 UTC 2012

Complementary phosphorylation sites in the adaptor protein SLP-76 promote synergistic activation of natural killer cells.

Science signaling

Kim HS,Long EO

Tue Jul 10 00:00:00 UTC 2012

Complementary phosphorylation sites in the adaptor protein SLP-76 promote synergistic activation of natural killer cells.

Science signaling

Kim HS,Long EO

Tue Jul 10 00:00:00 UTC 2012

Cell type-specific regulation of ITAM-mediated NF-kappaB activation by the adaptors, CARMA1 and CARD9.

Journal of immunology (Baltimore, Md. : 1950)

Hara H,Ishihara C,Takeuchi A,Xue L,Morris SW,Penninger JM,Yoshida H,Saito T

44-482 was used in western blot to study the impact of the caspase recruitment domain 9-Bcl10 complex on NK cell receptor signaling

Tue Jul 15 00:00:00 UTC 2008

Molecular dissection of 2B4 signaling: implications for signal transduction by SLAM-related receptors.

Molecular and cellular biology

Chen R,Relouzat F,Roncagalli R,Aoukaty A,Tan R,Latour S,Veillette A

Tue Jun 01 00:00:00 UTC 2004

生物信息学

蛋白别名: oncogene vav; Proto-oncogene vav; vav 1 guanine nucleotide exchange factor; vav 1 oncogene; vav proto-oncogene

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基因别名: VAV; VAV1

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UniProt ID: (Human) P15498

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Entrez Gene ID: (Human) 7409

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transcription factor activity, sequence-specific DNA binding guanyl-nucleotide exchange factor activity Rho guanyl-nucleotide exchange factor activity protein binding Rac guanyl-nucleotide exchange factor activity metal ion binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity

regulation of transcription, DNA-templated immune response G-protein coupled receptor signaling pathway integrin-mediated signaling pathway small GTPase mediated signal transduction regulation of cell size regulation of phosphatidylinositol 3-kinase signaling platelet activation T cell differentiation neutrophil chemotaxis T cell costimulation regulation of Rho protein signal transduction Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis positive regulation of apoptotic process regulation of GTPase activity positive regulation of GTPase activity positive regulation of natural killer cell mediated cytotoxicity phosphatidylinositol phosphorylation vascular endothelial growth factor receptor signaling pathway phosphatidylinositol-mediated signaling regulation of small GTPase mediated signal transduction reactive oxygen species metabolic process