Invitrogen

Phospho-VAV2 (Tyr142) Polyclonal Antibody

Phospho-VAV2 (Tyr142) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-VAV2 (Tyr142) Antibody (PA5-143787) in ICC/IF

Immunocytochemistry analysis of Phospho-VAV2 (Tyr142) in control and pervanadate-treated human A431 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40. Then labeled with rabbit Phospho-VAV2 (Tyr142) polyclonal antibody (Product # PA5-143787). The antibody was detected using goat anti-rabbit DyLight® 594.

Phospho-VAV2 (Tyr142) Antibody in Western Blot (WB)

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产品信息

PA5-143787

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Phospho-Vav2 (Tyr-142) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-142 in mouse Vav2. This sequence has high homology with similar regions in rat and human Vav2, and has significant homology to the conserved site in Vav1 and Vav3.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA, 50% glycerol

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2943015

产品详细信息

This antibody was cross-adsorbed to unphosphorylated Vav2 (Tyr-142) peptide before affinity purification using phospho-Vav2 (Tyr-142) peptide (without carrier). The antibody detects a 100 kDa* band corresponding to Vav on SDS-PAGE immunoblots of human A431 cells treated with EGF and detects Vav1, Vav2, and Vav3 in Jurkat cells treated with pervanadate. The antibody does not detect these Vavs after treatment with alkaline phosphatase.

靶标信息

VAV2 is the second member of the VAV guanine nucleotide exchange factor family of oncogenes. Unlike VAV1, which is expressed exclusively in hematopoietic cells, VAV2 transcripts were found in most tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。