Invitrogen

Phospho-VE-cadherin (Tyr731) Polyclonal Antibody

Phospho-VE-cadherin (Tyr731) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-VE-cadherin (Tyr731) Antibody (44-1145G) in ICC/IF

Immunofluorescence analysis of Phospho-VE-cadherin (Tyr731) was performed using 70% confluent log phase HUVEC cells treated with 100 ng/mL VEGF for 10 min. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-VE-cadherin (Tyr731) Polyclonal Antibody (Prod... View More

Phospho-VE-cadherin (Tyr731) Antibody in Western Blot (WB)

Phospho-VE-cadherin (Tyr731) Antibody in Immunohistochemistry (IHC)

Product Image Gallery

产品信息

44-1145G

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看12篇已发表文章 12篇已发表文章

免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

1:100

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其他PubMed (Misc)

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产品规格

种属反应

Human, Non-human primate

已发表种属

Hamster, Human, Mouse, Rat, Tag

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human VE-cadherin that contains tyrosine 731. This sequence is conserved in chimpanzee.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533584

靶标信息

VE-cadherin is a member of the cadherin superfamily that is located in a six-cadherin cluster in a region on the long arm of chromosome 16 and is involved in loss of heterozygosity events in breast and prostate cancer. VE-cadherin protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but the full length sequence of VE-cadherin has not been determined.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The short-chain fatty acid butyrate exerts a specific effect on VE-cadherin phosphorylation and alters the integrity of aortic endothelial cells.

Frontiers in cell and developmental biology

Guo J,Terhorst I,Stammer P,Ibrahim A,Oberhuber A,Eierhoff T

44-1145G was used in Western Blotting to suggest that BUT, an SCFA and gut microbiota-derived metabolite, impacts vascular integrity by targeting VEC phosphorylation with potential impact on the pathophysiology and therapy of vascular diseases.

Tue Feb 28 00:00:00 UTC 2023

The short-chain fatty acid butyrate exerts a specific effect on VE-cadherin phosphorylation and alters the integrity of aortic endothelial cells.

Frontiers in cell and developmental biology

Guo J,Terhorst I,Stammer P,Ibrahim A,Oberhuber A,Eierhoff T

Tue Feb 28 00:00:00 UTC 2023

SARS-CoV-2 spike spurs intestinal inflammation via VEGF production in enterocytes.

EMBO molecular medicine

Zeng FM,Li YW,Deng ZH,He JZ,Li W,Wang L,Lyu T,Li Z,Mei C,Yang M,Dong Y,Jiang GM,Li X,Huang X,Xiao F,Liu Y,Shan H,He H

Published figure using Phospho-VE-cadherin (Tyr731) polyclonal antibody (Product # 44-1145G) in Immunohistochemistry

Mon May 09 00:00:00 UTC 2022

GRP78 translocation to the cell surface and O-GlcNAcylation of VE-Cadherin contribute to ER stress-mediated endothelial permeability.

Scientific reports

Lenin R,Nagy PG,Jha KA,Gangaraju R

44-1145G was used in Western Blotting to test the hypothesis that increased ER stress via O-GlcNAcylation of VE-Cadherin likely contribute to endothelial permeability.

Thu Jul 25 00:00:00 UTC 2019

Time-Variant SRC Kinase Activation Determines Endothelial Permeability Response.

Cell chemical biology

Klomp JE,Shaaya M,Matsche J,Rebiai R,Aaron JS,Collins KB,Huyot V,Gonzalez AM,Muller WA,Chew TL,Malik AB,Karginov AV

44-1145G was used in Western Blot to control tyrosine kinase SRC activation to investigate endothelial barrier enhancement via SRC required phosphorylation of VE cadherin at Y731.

Thu Aug 15 00:00:00 UTC 2019

Rap1B promotes VEGF-induced endothelial permeability and is required for dynamic regulation of the endothelial barrier.

Journal of cell science

Lakshmikanthan S,Sobczak M,Li Calzi S,Shaw L,Grant MB,Chrzanowska-Wodnicka M

44-1145G was used in Western Blotting to determine the role of Rap1 in endothelial barrier dynamics.

Wed Jan 10 00:00:00 UTC 2018

Deubiquitinase function of A20 maintains and repairs endothelial barrier after lung vascular injury.

Cell death discovery

Soni D,Wang DM,Regmi SC,Mittal M,Vogel SM,Schlüter D,Tiruppathi C

Thu Oct 22 00:00:00 UTC 2020

Annexin A2 supports pulmonary microvascular integrity by linking vascular endothelial cadherin and protein tyrosine phosphatases.

The Journal of experimental medicine

Luo M,Flood EC,Almeida D,Yan L,Berlin DA,Heerdt PM,Hajjar KA

Published figure using Phospho-VE-cadherin (Tyr731) polyclonal antibody (Product # 44-1145G) in Western Blot

Mon Sep 04 00:00:00 UTC 2017

Pyk2 phosphorylation of VE-PTP downstream of STIM1-induced Ca²⁺ entry regulates disassembly of adherens junctions.

American journal of physiology. Lung cellular and molecular physiology

Soni D,Regmi SC,Wang DM,DebRoy A,Zhao YY,Vogel SM,Malik AB,Tiruppathi C

441145G was used in western blot to determine the role of STIM1 activation of store-operated Ca2+ entry in regulating adherens junction assembly

Thu Jun 01 00:00:00 UTC 2017

Endothelial Gαq/11 is required for VEGF-induced vascular permeability and angiogenesis.

Cardiovascular research

Sivaraj KK,Li R,Albarran-Juarez J,Wang S,Tischner D,Grimm M,Swiercz JM,Offermanns S,Wettschureck N

44-1145G was used in immunohistochemistry to assess the role of Gαq/11 in VEGF-dependent regulation of vascular permeability and angiogenesis.

Thu Oct 01 00:00:00 UTC 2015

Novel role of CD47 in rat microvascular endothelium: signaling and regulation of T-cell transendothelial migration.

Arteriosclerosis, thrombosis, and vascular biology

Martinelli R,Newton G,Carman CV,Greenwood J,Luscinskas FW

Published figure using Phospho-VE-cadherin (Tyr731) polyclonal antibody (Product # 44-1145G) in Western Blot

Fri Nov 01 00:00:00 UTC 2013

Metabotropic glutamate receptor 5 mediates phosphorylation of vascular endothelial cadherin and nuclear localization of β-catenin in response to homocysteine.

Vascular pharmacology

Beard RS,Reynolds JJ,Bearden SE

Tue Jul 17 00:00:00 UTC 2012

Metabotropic glutamate receptor 5 mediates phosphorylation of vascular endothelial cadherin and nuclear localization of β-catenin in response to homocysteine.

Vascular pharmacology

Beard RS,Reynolds JJ,Bearden SE

Tue Jul 17 00:00:00 UTC 2012

Metabotropic glutamate receptor 5 mediates phosphorylation of vascular endothelial cadherin and nuclear localization of β-catenin in response to homocysteine.

Vascular pharmacology

Beard RS,Reynolds JJ,Bearden SE

Tue Jul 17 00:00:00 UTC 2012

Metabotropic glutamate receptor 5 mediates phosphorylation of vascular endothelial cadherin and nuclear localization of β-catenin in response to homocysteine.

Vascular pharmacology

Beard RS,Reynolds JJ,Bearden SE

Tue Jul 17 00:00:00 UTC 2012

Annexin 2 regulates endothelial morphogenesis by controlling AKT activation and junctional integrity.

The Journal of biological chemistry

Su SC,Maxwell SA,Bayless KJ

44-1145G was used in western blot to identify annexin 2 as a regulator of endothelial morphogenesis

Fri Dec 24 00:00:00 UTC 2010

Annexin 2 regulates endothelial morphogenesis by controlling AKT activation and junctional integrity.

The Journal of biological chemistry

Su SC,Maxwell SA,Bayless KJ

44-1145G was used in western blot to identify annexin 2 as a regulator of endothelial morphogenesis

Fri Dec 24 00:00:00 UTC 2010

ICAM-1-mediated, Src- and Pyk2-dependent vascular endothelial cadherin tyrosine phosphorylation is required for leukocyte transendothelial migration.

Journal of immunology (Baltimore, Md. : 1950)

Allingham MJ,van Buul JD,Burridge K

Sat Sep 15 00:00:00 UTC 2007

ICAM-1-mediated, Src- and Pyk2-dependent vascular endothelial cadherin tyrosine phosphorylation is required for leukocyte transendothelial migration.

Journal of immunology (Baltimore, Md. : 1950)

Allingham MJ,van Buul JD,Burridge K

Sat Sep 15 00:00:00 UTC 2007

Tyrosine phosphorylation of VE-cadherin prevents binding of p120- and beta-catenin and maintains the cellular mesenchymal state.

The Journal of biological chemistry

Potter MD,Barbero S,Cheresh DA

Fri Sep 09 00:00:00 UTC 2005

Tyrosine phosphorylation of VE-cadherin prevents binding of p120- and beta-catenin and maintains the cellular mesenchymal state.

The Journal of biological chemistry

Potter MD,Barbero S,Cheresh DA

Fri Sep 09 00:00:00 UTC 2005

生物信息学

蛋白别名: 7B4 antigen; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144; cd144 antigen; endothelial-specific cadherin; Vascular endothelial cadherin; VE-cadherin

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基因别名: 7B4; CD144; CDH5

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UniProt ID: (Human) P33151

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Entrez Gene ID: (Human) 1003

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功能

receptor binding calcium ion binding protein binding beta-catenin binding protein phosphatase binding ion channel binding cadherin

参与通路

blood vessel maturation cell-cell junction assembly homophilic cell adhesion via plasma membrane adhesion molecules transforming growth factor beta receptor signaling pathway negative regulation of cell proliferation single organismal cell-cell adhesion adherens junction organization positive regulation of establishment of endothelial barrier regulation of establishment of cell polarity