Invitrogen

Phospho-VEGF Receptor 2 (Tyr1214) Polyclonal Antibody

Phospho-VEGF Receptor 2 (Tyr1214) Antibody in Western Blot (WB)

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Phospho-VEGF Receptor 2 (Tyr1214) Antibody (44-1052) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of HT-29 (Lane 1), HT-29 Serum Starved followed by treatment for 10 minutes with 100 ng/mL of SCF (Lane 2), CaCo-2 (Lane 3) and CaCo-2 Serum Starved followed by treatment for 10 minutes with 100 ng/mL of SCF (Lane 4). The blots were probed with Anti-VEGF Receptor 2 (pY1214) Rabbit Polyclonal Antibody (Product # 44-1052, 1:1000 dilutio... View More

Phospho-VEGF Receptor 2 (Tyr1214) Antibody in Immunocytochemistry (ICC/IF)

Phospho-VEGF Receptor 2 (Tyr1214) Antibody

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产品信息

44-1052

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫细胞化学 (ICC/IF)

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其他PubMed (Misc)

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human VEGFR2 that contains tyrosine 1214. The sequence is conserved in mouse and rat.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533558

靶标信息

KDR (FLK1, VEGFR2) is a type III receptor tyrosine kinase. It binds vascular endothelial growth factor with high affinity in vitro, and is expressed early in development by endothelial cell precursors. FLK1 (VEGF Receptor 2) encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. Mutations of FLK1 (VEGF Receptor 2) are implicated in infantile capillary hemangiomas.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Blockade of insulin receptor substrate-1 inhibits biological behavior of choroidal endothelial cells.

International journal of ophthalmology

Qian YY,Wu HY,Liu GQ,Ren C,Lu PR,Zhang XG

44-1052 was used in Western Blotting to investigate the effects of blockade of insulin receptor substrate-1 (IRS-1) on the bio-function of tube formation of human choroidal endothelial cells (HCECs).

Wed Sep 30 00:00:00 UTC 2020

Epidermal-specific deletion of TC-PTP promotes UVB-induced epidermal cell survival through the regulation of Flk-1/JNK signaling.

Cell death & disease

Baek M,Kim M,Lim JS,Morales LD,Hernandez J,Mummidi S,Williams-Blangero S,Jang IS,Tsin AT,Kim DJ

44-1052 was used in Western Blotting to suggest that T-cell protein tyrosine phosphatase is protective against UVB-induced keratinocyte cell damage by promoting apoptosis via negative regulation of Flk-1/JNK survival signalling.

Thu Jun 28 00:00:00 UTC 2018

The docking protein FRS2α is a critical regulator of VEGF receptors signaling.

Proceedings of the National Academy of Sciences of the United States of America

Chen PY,Qin L,Zhuang ZW,Tellides G,Lax I,Schlessinger J,Simons M

44-1052 was used in western blot to show that FRS2α is a component of the VEGF receptors.

Tue Apr 15 00:00:00 UTC 2014

The docking protein FRS2α is a critical regulator of VEGF receptors signaling.

Proceedings of the National Academy of Sciences of the United States of America

Chen PY,Qin L,Zhuang ZW,Tellides G,Lax I,Schlessinger J,Simons M

44-1052 was used in western blot to show that FRS2α is a component of the VEGF receptors.

Tue Apr 15 00:00:00 UTC 2014

Microtubules coordinate VEGFR2 signaling and sorting.

PloS one

Czeisler C,Mikawa T

44-1052 was used in western blot to study intracellular sorting of VEGFR2 after ligand stimulation

Tue May 27 00:00:00 UTC 2014

The important roles of RET, VEGFR2 and the RAF/MEK/ERK pathway in cancer treatment with sorafenib.

Acta pharmacologica Sinica

Mao WF,Shao MH,Gao PT,Ma J,Li HJ,Li GL,Han BH,Yuan CG

Mon Oct 01 00:00:00 UTC 2012

Synthetic heparan sulfate oligosaccharides inhibit endothelial cell functions essential for angiogenesis.

PloS one

Cole CL,Hansen SU,Baráth M,Rushton G,Gardiner JM,Avizienyte E,Jayson GC

44-1052 was used in western blot to study heparan sulfate regulated cytokine-dependent angiogenic cellular functions

Wed Jul 21 00:00:00 UTC 2010

Synthetic heparan sulfate oligosaccharides inhibit endothelial cell functions essential for angiogenesis.

PloS one

Cole CL,Hansen SU,Baráth M,Rushton G,Gardiner JM,Avizienyte E,Jayson GC

44-1052 was used in western blot to study heparan sulfate regulated cytokine-dependent angiogenic cellular functions

Wed Jul 21 00:00:00 UTC 2010

Synthetic heparan sulfate oligosaccharides inhibit endothelial cell functions essential for angiogenesis.

PloS one

Cole CL,Hansen SU,Baráth M,Rushton G,Gardiner JM,Avizienyte E,Jayson GC

44-1052 was used in western blot to study heparan sulfate regulated cytokine-dependent angiogenic cellular functions

Wed Jul 21 00:00:00 UTC 2010

生物信息学

蛋白别名: CD309; CD309 antigen; Fetal liver kinase 1; fetal liver kinase-1; FLK-1; KDR; Kinase insert domain receptor; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase NYK; Protein-tyrosine kinase receptor flk-1; sCD309; soluble CD309; soluble vascular endothelial growth factor receptor 2; soluble VEGFR 2; soluble VEGFR2; tyrosine kinase growth factor receptor; Vascular endothelial growth factor receptor 2; vascular endothelial growth factor receptor- 2; vascular endothelial growth factor receptor-2; vascular endothelial growth factor receptor-3; VEGF R2; VEGF receptor-2; VEGFR-2

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基因别名: 6130401C07; CD309; Flk-1; FLK1; KDR; Krd-1; Ly73; sVEGFR-2; VEGFR; VEGFR-2; VEGFR2

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UniProt ID: (Human) P35968, (Mouse) P35918

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Entrez Gene ID: (Human) 3791, (Mouse) 16542

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功能