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Phospho-Vinculin (Tyr1133, Tyr1065) Antibody (PA5-106113) in ICC/IF
Immunofluorescent analysis of Phospho-Vinculin (Tyr1133, Tyr1065) in HeLa cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-Vinculin (Tyr1133, Tyr1065) polyclonal antibody (Product # PA5-106113) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti... View More
产品信息
PA5-106113
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human VCL(Accession P18206), corresponding to amino acid residues around phosphorylated Tyr1133.
偶联物
形式
Liquid
浓度
1 mg/mL
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2817512
产品详细信息
Antibody detects endogenous levels of Vinculin only when phosphorylated at Tyr1133(Isoform1,P18206-2) or Tyr1065(Isoform2,P18206-1).
靶标信息
Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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