Invitrogen

Phospho-YAP1 (Ser127) Polyclonal Antibody

Phospho-YAP1 (Ser127) Antibody in Western Blot (WB)

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Phospho-YAP1 (Ser127) Antibody (PA5-17481) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), MDA-MB-231 treated with Forskolin (10 µM for 60 min) (Lane 2), PC-3 (Lane 3), PC-3 treated with Forskolin (10 µM for 60 min) and U-87 MG (Lane 5). The blot was probed with Anti-Phospho-YAP1 (Ser127) Polyclonal Antibody (Product # PA5-17481, 1:1,000 dilution) and detected by chemiluminescence using Goat anti-Rab... View More

Phospho-YAP1 (Ser127) Antibody in Immunocytochemistry (ICC/IF)

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产品信息

PA5-17481

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫细胞化学 (ICC/IF)

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免疫沉淀 (IP)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic phosphopeptide corresponding to residues surrounding pSer127 of human YAP

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

59.2 µg/mL

纯化类型

Antigen affinity chromatography, Protein A

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10981511

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

YAP (Yes-Associated Protein) is a founding member of the WW domain family of proteins, transcriptional co-activator and oncogene. Its gene is transcriptionally activated in squamous cell carcinoma cells. YAP can act both as a coactivator and corepressor and is the critical downstream regulatory target in the Hippo signaling pathway. The Hippo signaling pathway plays a pivotal role in organ size control and tumour suppression by restricting proliferation and promoting apoptosis. Also plays key roles in tissue tension, 3D tissue shape, cell proliferation, cell death, and cell migration. Mutations in the IRF5 gene results in coloboma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Biglycan and reduced glycolysis are associated with breast cancer cell dormancy in the brain.

Frontiers in oncology

Sunderland A,Williams J,Andreou T,Rippaus N,Fife C,James F,Kartika YD,Speirs V,Carr I,Droop A,Lorger M

Published figure using Phospho-YAP1 (Ser127) polyclonal antibody (Product # PA5-17481) in Western Blot

Tue Jul 18 00:00:00 UTC 2023

Novel ROCK Inhibitors, Sovesudil and PHP-0961, Enhance Proliferation, Adhesion and Migration of Corneal Endothelial Cells.

International journal of molecular sciences

Kim KW,Shin YJ,Lee SCS

Published figure using Phospho-YAP1 (Ser127) polyclonal antibody (Product # PA5-17481) in Western Blot

Thu Nov 24 00:00:00 UTC 2022

Acute normobaric hypoxia blunts contraction-mediated mTORC1- and JNK-signaling in human skeletal muscle.

Acta physiologica (Oxford, England)

Moberg M,Apró W,Horwath O,van Hall G,Blackwood SJ,Katz A

PA5-17481 was used in Western Blotting to examine the effect of acute hypoxia on the activity of several signalling pathways involved in the regulation of muscle growth following a bout of resistance exercise.

Tue Feb 01 00:00:00 UTC 2022

The aryl hydrocarbon receptor promotes differentiation during mouse preimplantational embryo development.

Stem cell reports

Nacarino-Palma A,González-Rico FJ,Rejano-Gordillo CM,Ordiales-Talavero A,Merino JM,Fernández-Salguero PM

PA5-17481 was used in Immunocytochemistry to present that AhR is a regulator of pluripotency and differentiation in early mouse embryogenesis.

Tue Sep 14 00:00:00 UTC 2021

The aryl hydrocarbon receptor promotes differentiation during mouse preimplantational embryo development.

Stem cell reports

Nacarino-Palma A,González-Rico FJ,Rejano-Gordillo CM,Ordiales-Talavero A,Merino JM,Fernández-Salguero PM

PA5-17481 was used in Immunocytochemistry to present that AhR is a regulator of pluripotency and differentiation in early mouse embryogenesis.

Tue Sep 14 00:00:00 UTC 2021

Livin promotes tumor progression through YAP activation in ovarian cancer.

American journal of cancer research

Gao J,Han W,He Y,Zhou J,Miao J,Zhang G

PA5-17481 was used in Western Blotting to reveal that inhibition of YAP by short-hairpin RNA prevents the growth and invasion of ovarian cancer cells in vivo and in vitro.

Thu Nov 12 00:00:00 UTC 2020

FAT4 silencing promotes epithelial-to-mesenchymal transition and invasion via regulation of YAP and β-catenin activity in ovarian cancer.

BMC cancer

Malgundkar SH,Burney I,Al Moundhri M,Al Kalbani M,Lakhtakia R,Okamoto A,Tamimi Y

PA5-17481 was used in Western Blotting to examine the role of FAT4 in proliferation and metastasis, and its mechanisms of interaction in these processes.

Mon May 04 00:00:00 UTC 2020

Targeting TEAD/YAP-transcription-dependent necrosis, TRIAD, ameliorates Huntington's disease pathology.

Human molecular genetics

Mao Y,Chen X,Xu M,Fujita K,Motoki K,Sasabe T,Homma H,Murata M,Tagawa K,Tamura T,Kaye J,Finkbeiner S,Blandino G,Sudol M,Okazawa H

PA5-17481 was used in Immunoprecipitation to suggest approaches targeting the signalling pathway of TEAD/YAP-transcription-dependent necrosis (TRIAD) could lead to a therapeutic development against HD.

Tue Nov 01 00:00:00 UTC 2016

生物信息学

蛋白别名: 65 kDa Yes-associated protein; Protein yorkie homolog; Transcriptional coactivator YAP1; Yes-associated protein 1; yes-associated protein 2; Yes-associated protein YAP65 homolog; yes-associated protein, 65 kDa; yorkie homolog

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基因别名: AI325207; COB1; YAP; YAP1; YAP2; YAP65; YKI; Yorkie

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UniProt ID: (Human) P46937, (Mouse) P46938

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Entrez Gene ID: (Human) 10413, (Rat) 363014, (Mouse) 22601

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