Invitrogen

Phospho-eNOS (Ser1177) Monoclonal Antibody (H.83.2)

Phospho-eNOS (Ser1177) Antibody in Western Blot (WB)

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Phospho-eNOS (Ser1177) Antibody (MA5-14957) in WB

Western blot was performed using Anti-Phospho-eNOS (Ser1177) Monoclonal Antibody (H.83.2) (Product # MA5-14957) and a 160 kDa band corresponding to Phospho-eNOS (Ser1177) was observed to be upregulated upon treatment with VEGF (10 ng/mL for 60 min) and H2O2 (300 µM for 30 min). Whole cell extracts (30 µg lysate) of HAEC (Lane 1), HAEC treated with VEGF (10 ng/mL for 60 min) (Lane 2), HUVEC (Lane 3), HUVEC t... View More

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产品信息

MA5-14957

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看6篇已发表文章 6篇已发表文章

免疫细胞化学 (ICC/IF)

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

1:25

产品规格

种属反应

Bovine, Human, Pig

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Monoclonal

类型

Antibody

克隆号

H.83.2

抗原

Synthetic phosphopeptide corresponding to residues surrounding pSer1177 of human eNOS

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

116 µg/mL

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

<0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_10984344

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Blocking cholesterol formation and turnover improves cellular and mitochondria function in murine heart microvascular endothelial cells and cardiomyocytes.

Frontiers in physiology

Braczko A,Harasim G,Kawecka A,Walczak I,Kapusta M,Narajczyk M,Stawarska K,Smoleński RT,Kutryb-Zając B

MA5-14957 was used in Immunocytochemistry to demonstrate that both treatments benefited the endothelial cell and cardiomyocyte bioenergetics, but the effects of PCSK9i were superior.

Tue Sep 26 00:00:00 UTC 2023

Protective effects of endothelial progenitor cell microvesicles carrying miR‑98‑5p on angiotensin II‑induced rat kidney cell injury.

Experimental and therapeutic medicine

Mai H,Huang Z,Zhang X,Zhang Y,Chen J,Chen M,Zhang Y,Song Y,Wang B,Lin Y,Gu S

MA5-14957 was used in Western Blotting to demonstrate that EPC-MVs with high expression of miR-98-5p can activate the PI3K/Akt/eNOS pathway by regulating IGF1R, as well as protect PRKs from Ang II-induced oxidative stress, inflammation and inhibition of cell viability.

Tue Nov 01 00:00:00 UTC 2022

Endurance Exercise Training-Attenuated Diabetic Kidney Disease with Muscle Weakness in Spontaneously Diabetic Torii Fatty Rats.

Kidney & blood pressure research

Kotake H,Yamada S,Ogura Y,Watanabe S,Inoue K,Ichikawa D,Sugaya T,Ohata K,Natsuki Y,Hoshino S,Watanabe M,Kimura K,Shibagaki Y,Kamijo-Ikemori A

MA5-14957 was used in Western Blot to evaluate protective effects of endurance exercise training against diabetic kidney disease (DKD) with muscle weakness by using male spontaneously diabetic Torii (SDT) fatty rats as type 2 diabetic animal models with obesity, hypertension, and hyperlipidemia.

Wed Mar 16 00:00:00 UTC 2022

Peroxidasin promotes diabetic vascular endothelial dysfunction induced by advanced glycation end products via NOX2/HOCl/Akt/eNOS pathway.

Redox biology

Zhang G,Liu Z,Xu Q,Li C,Cheng G,Shi R

MA5-14957 was used in Western Blot to elucidate the role of peroxidasin in promoting vascular endothelial dysfunction induced by advanced glycation end products in diabetes mellitus.

Wed Sep 01 00:00:00 UTC 2021

Peroxidasin promotes diabetic vascular endothelial dysfunction induced by advanced glycation end products via NOX2/HOCl/Akt/eNOS pathway.

Redox biology

Zhang G,Liu Z,Xu Q,Li C,Cheng G,Shi R

MA5-14957 was used in Western Blot to elucidate the role of peroxidasin in promoting vascular endothelial dysfunction induced by advanced glycation end products in diabetes mellitus.

Wed Sep 01 00:00:00 UTC 2021

Fibroblast growth factor 21 Ameliorates diabetes-induced endothelial dysfunction in mouse aorta via activation of the CaMKK2/AMPKα signaling pathway.

Cell death & disease

Ying L,Li N,He Z,Zeng X,Nan Y,Chen J,Miao P,Ying Y,Lin W,Zhao X,Lu L,Chen M,Cen W,Guo T,Li X,Huang Z,Wang Y

MA5-14957 was used in Western Blotting to study the effect of FGF21 on endothelial function in both type 1 and type 2 diabetes.

Wed Sep 11 00:00:00 UTC 2019

IGF-1 receptor cleavage in hypertension.

Hypertension research : official journal of the Japanese Society of Hypertension

Cirrik S,Schmid-Schönbein GW

MA5-14957 was used in Western Blotting to investigate the association of an enhanced protease activity and IGF-1 receptor cleavage using the spontaneously hypersensitive rats and their normotensive Wistar Kyoto controls.

Fri Jun 01 00:00:00 UTC 2018

Telomerase reverse transcriptase protects against angiotensin II-induced microvascular endothelial dysfunction.

American journal of physiology. Heart and circulatory physiology

Ait-Aissa K,Kadlec AO,Hockenberry J,Gutterman DD,Beyer AM

Published figure using Phospho-eNOS (Ser1177) monoclonal antibody (Product # MA5-14957) in Western Blot

Tue May 01 00:00:00 UTC 2018

生物信息学

蛋白别名: cNOS; Constitutive NOS; e-NOS; EC-NOS; endothelial nitric oxide synthase; Endothelial NOS; eNOS; Nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; Nitric oxide synthase, endothelial; NOS type III; NOSIII; OTTHUMP00000213183

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基因别名: cNOS; EC-NOS; ECNOS; eNOS; NOS; NOS3; NOSIII

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UniProt ID: (Pig) Q28969, (Human) P29474

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Entrez Gene ID: (Bovine) 287024, (Pig) 397557, (Human) 4846

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功能