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Phospho-iNOS (Tyr1055) Antibody (PA5-143731) in ICC/IF
Immunocytochemistry analysis of Phospho-iNOS (Tyr1055) in rat PC12 cells differentiated with NGF. The cells were probed with mouse monoclonal nNOS, and rabbit polyclonal nNOS (C-terminal region), Phospho-eNOS/nNOS (Tyr657, Tyr895) polyclonal antibody (Product # PA5-143729), and Phospho-iNOS (Tyr1055) polyclonal antibody (Product # PA5-143731). The antibodies were detected using appropriate secondary antibod... View More
产品信息
PA5-143731
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Phospho-iNOS (Tyr-1055) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Tyr-1055 in human iNOS. This sequence is well conserved in rat and mouse iNOS, and has some homology to the conserved sites in eNOS (Tyr-1087) and nNOS (Tyr-1326).
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA, 50% glycerol
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2942959
产品详细信息
The antibody detects a 130 kDa* protein corresponding to induced NOS on SDS-PAGE immunoblots of mouse macrophages treated with LPS followed by pervanadate. The antibody also detects a 150 kDa doublet that corresponds with higher molecular weight forms of NOS, which are also observed using other anti-iNOS antibodies. In addition, the antibody detects a non-specific 120 kDa band that has not been identified.
靶标信息
iNOS (Inducible Nitric oxide, NO, NOS) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. iNOS is expressed in liver and inducible by a combination of lipopolysaccharide and certain cytokines. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. Diseases associated with iNOS dysfunction include achalasia and impotence.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: HEP-NOS; Hepatocyte NOS; hepatocytes; inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; MAC-NOS; Macrophage NOS; nitric oxide synthase 2, inducible; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase 2A (inducible, hepatocytes); Nitric oxide synthase, inducible; nitric oxide synthase, macrophage; nitric oxide synthase-inducible; NOS type II; NOS, type II; OTTMUSP00000000202; Peptidyl-cysteine S-nitrosylase NOS2
基因别名: HEP-NOS; i-NOS; INOS; Inosl; NOS; Nos-2; NOS-II; NOS2; NOS2A
UniProt ID: (Human) O94994, (Mouse) P29477, (Rat) Q06518
Entrez Gene ID: (Human) 4843, (Mouse) 18126, (Rat) 24599
nitric-oxide synthase activity
receptor binding
protein binding
calmodulin binding
FMN binding
heme binding
tetrahydrobiopterin binding
arginine binding
protein homodimerization activity
metal ion binding
flavin adenine dinucleotide binding
NADP binding
actin binding
NADPH-hemoprotein reductase activity
iron ion binding
beta-catenin binding
cAMP-dependent protein kinase regulator activity
oxidoreductase activity
protein kinase binding
cadherin binding
nitric-oxide synthase binding
Hsp90 protein binding
amino acid binding
oxidoreductase
protein modifying enzyme
response to hypoxia
positive regulation of leukocyte mediated cytotoxicity
innate immune response in mucosa
arginine catabolic process
superoxide metabolic process
nitric oxide biosynthetic process
nitric oxide mediated signal transduction
circadian rhythm
response to bacterium
negative regulation of gene expression
peptidyl-cysteine S-nitrosylation
positive regulation of guanylate cyclase activity
prostaglandin secretion
cellular response to drug
regulation of cell proliferation
negative regulation of protein catabolic process
defense response to bacterium
regulation of cellular respiration
cell redox homeostasis
negative regulation of blood pressure
positive regulation of vasodilation
regulation of insulin secretion
defense response to Gram-negative bacterium
positive regulation of killing of cells of other organism
oxidation-reduction process
cellular response to lipopolysaccharide
cellular response to interferon-gamma
interleukin-6 secretion
interleukin-8 secretion
regulation of cytokine production involved in inflammatory response
ovulation from ovarian follicle
inflammatory response
response to lipopolysaccharide
regulation of protein kinase activity
endothelial cell proliferation
blood vessel remodeling
signal transduction
G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger
aging
regulation of heart contraction
regulation of blood pressure
response to mechanical stimulus
response to hormone
response to activity
glycoside metabolic process
cGMP-mediated signaling
response to estradiol
response to tumor necrosis factor
intracellular signal transduction
response to immobilization stress
wound healing
positive regulation of apoptotic process
retinoic acid receptor signaling pathway
cellular response to lithium ion
cellular response to vitamin D
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular response to redox state
response to dexamethasone
