Invitrogen

Phospho-p47phox (Ser370) Polyclonal Antibody

Phospho-p47phox (Ser370) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Phospho-p47phox (Ser370) Antibody (PA5-36863) in IHC (P)

Immunohistochemistry analysis of Phospho-p47phox (Ser370) in paraffin-embedded human tonsil carcinoma tissue (cytoplasmic and nucleus staining) and negative control (right, with PBS only). Samples were incubated with Phospho-p47phox (Ser370) polyclonal antibody (Product # PA5-36863) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.

Phospho-p47phox (Ser370) Antibody in Western Blot (WB)

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产品信息

PA5-36863

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

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免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫组化（冰冻） (IHC (F))

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免疫细胞化学 (ICC/IF)

1:50-1:200

免疫沉淀 (IP)

1:50-1:200

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Bovine, Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic phosphopeptide derived from human p47-phox around the phosphorylation site of Serine 370

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 50% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2553788

产品详细信息

This antibody detects endogenous protein at a molecular weight of 45 kDa.

Purity is >95% by SDS-PAGE.

靶标信息

p47phox (NCF1) is required, along with NCF2 and membrane bound cytochrome b558, for activation of the latent NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in the gene can result in granulomatous disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The metabolic function of pyruvate kinase M2 regulates reactive oxygen species production and microbial killing by neutrophils.

Nature communications

Toller-Kawahisa JE,Hiroki CH,Silva CMS,Nascimento DC,Públio GA,Martins TV,Damasceno LEA,Veras FP,Viacava PR,Sukesada FY,Day EA,Zotta A,Ryan TAJ,Moreira da Silva R,Cunha TM,Lopes NP,Cunha FQ,O'Neill LAJ,Alves-Filho JC

PA5-36863 was used in Western Blotting to report that PKM2 regulates ROS production and microbial killing by neutrophils.

Mon Jul 17 00:00:00 UTC 2023

Distinct histopathological phenotypes of severe alcoholic hepatitis suggest different mechanisms driving liver injury and failure.

The Journal of clinical investigation

Ma J,Guillot A,Yang Z,Mackowiak B,Hwang S,Park O,Peiffer BJ,Ahmadi AR,Melo L,Kusumanchi P,Huda N,Saxena R,He Y,Guan Y,Feng D,Sancho-Bru P,Zang M,Cameron AM,Bataller R,Tacke F,Sun Z,Liangpunsakul S,Gao B

PA5-36863 was used in Immunohistochemistry-immunofluorescence to conclude that two distinct histopathological phenotypes based on liver immune phenotyping are observed in SAH patients, suggesting a separate mechanism driving liver injury and/or failure in these patients.

Fri Jul 15 00:00:00 UTC 2022

Zinc regulates reactive oxygen species generation in platelets.

Platelets

Lopes-Pires ME,Ahmed NS,Vara D,Gibbins JM,Pula G,Pugh N

PA5-36863 was used in Western Blotting to investigate the relationship between fluctuations in cytosolic Zn2 [Zn2+]i and platelet ROS production.

Sat Apr 03 00:00:00 UTC 2021

Vitamin D receptor activation regulates microglia polarization and oxidative stress in spontaneously hypertensive rats and angiotensin II-exposed microglial cells: Role of renin-angiotensin system.

Redox biology

Cui C,Xu P,Li G,Qiao Y,Han W,Geng C,Liao D,Yang M,Chen D,Jiang P

Published figure using Phospho-p47phox (Ser370) polyclonal antibody (Product # PA5-36863) in Western Blot

Sun Sep 01 00:00:00 UTC 2019

Role of PLD-PKCζ signaling axis in p47phox phosphorylation for activation of NADPH oxidase by angiotensin II in pulmonary artery smooth muscle cells.

Cell biology international

Chakraborti S,Sarkar J,Chakraborti T

PA5-36863 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to conclude that PLD-PKCζ-p47phox signaling axis plays a crucial role in ANGII-induced increase in NADPH oxidase-mediated O2.- production in the cells.

Sat Jun 01 00:00:00 UTC 2019

生物信息学

蛋白别名: 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NADPH oxidase organizer 2; NADPH oxidase subunit (47 kD); NADPH oxidase subunit (47kDa); NCF-1; NCF-47K; neutrop; Neutrophil cytosol factor 1; neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1); Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; P47 phox; p47-phox; phagocyte oxidase 47 kDa; SH3 and PX domain-containing protein 1A

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基因别名: Ncf-1; NCF-47K; NCF1; NCF1A; NOXO2; p47-phox; p47; p47phox; SH3PXD1A

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UniProt ID: (Human) P14598, (Mouse) Q09014

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Entrez Gene ID: (Human) 653361, (Mouse) 17969, (Rat) 114553

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功能

protein binding electron carrier activity superoxide-generating NADPH oxidase activity superoxide-generating NADPH oxidase activator activity SH3 domain binding phosphatidylinositol binding phosphatidylinositol-3,4-bisphosphate binding lipid binding

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