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Platelet-activating factor receptor (extracellular) Polyclonal Antibody

查看其他7个PTAFR抗体

抗体检测数据 (4)

Platelet-activating factor receptor (extracellular) Antibody in Immunohistochemistry (Frozen) (IHC (F))

Platelet-activating factor receptor (extracellular) Antibody in Immunohistochemistry (Frozen) (IHC (F))

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Platelet-activating factor receptor (extracellular) Antibody (APR-055-200UL) in IHC (F)

Expression of Platelet-activating factor receptor in rat substantia nigra pars compacta (SNC). Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Platelet-activating factor receptor (extracellular) Antibody (#APR-055), (1:300), followed by goat Anti-rabbit-AlexaFluor-488. A. PAFR immunoreactivity (green) appears in SNC neurons (arrows). B. Pre-incubation of the Antibody with... View More

Platelet-activating factor receptor (extracellular) Antibody in Immunohistochemistry (Frozen) (IHC (F))

Platelet-activating factor receptor (extracellular) Antibody in Western Blot (WB)

Platelet-activating factor receptor (extracellular) Antibody in Western Blot (WB)

Platelet-activating factor receptor (extracellular) Antibody in Flow Cytometry (Flow)

Product Image Gallery

Platelet-activating factor receptor (extracellular) Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Expression of Platelet-activating factor receptor in rat substantia nigra pars compacta (SNC). Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Platelet-activating factor receptor (extracellular) Antibody (#APR-055), (1:300), followed by goat Anti-rabbit-AlexaFluor-488. A. PAFR immunoreactivity (green) appears in SNC neurons (arrows). B. Pre-incubation of the Antibody with Platelet-activating factor receptor (extracellular) Blocking Peptide (BLP-PR055), suppressed staining. Cell nuclei are stained with DAPI (blue).

Platelet-activating factor receptor (extracellular) Antibody in Western Blot (WB) — Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human MEG-01 megakaryoblastic leukemia cell line lysate (lanes 2 and 4): - 1-2. Anti-Platelet-activating factor receptor (extracellular) Antibody (#APR-055), (1:500). 3-4. Anti-Platelet-activating factor receptor (extracellular) Antibody , preincubated with Platelet-activating factor receptor (extracellular) Blocking Peptide (BLP-PR055).

Platelet-activating factor receptor (extracellular) Antibody in Western Blot (WB) — Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 1-2. Anti-Platelet-activating factor receptor (extracellular) Antibody (#APR-055), (1:500). 3-4. Anti-Platelet-activating factor receptor (extracellular) Antibody , preincubated with Platelet-activating factor receptor (extracellular) Blocking Peptide (BLP-PR055).

Platelet-activating factor receptor (extracellular) Antibody in Flow Cytometry (Flow) — Cell surface detection of PAFR by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-Platelet-activating factor receptor (extracellular) Antibody (#APR-055) (2.5μg) + goat- Anti-rabbit-FITC.

产品信息

APR-055-200UL

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

-

免疫组化（冰冻） (IHC (F))

1:300

-

流式细胞分析 (Flow)

2.5 µg

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

(C)ELGYQTNFHQAINDAH, corresponding to amino acid residues 259-274 of mouse PTAFR, Extracellular, 3rd loop

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.8 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 1% BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 X g 5 min).

靶标信息

PTAFR shows structural characteristics of the rhodopsin gene family and binds platelet-activating factor (PAF). PAF is a phospholipid (1-0-alkyl-2-acetyl-sn-glycero-3-phosphorylcholine) that has been implicated as a mediator in diverse pathologic processes, such as allergy, asthma, septic shock, arterial thrombosis, and inflammatory processes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: PAF receptor; PAF-R; PAFr; Platelet-activating factor receptor

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基因别名: PAFR; PTAFR

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UniProt ID: (Human) P25105, (Mouse) Q62035, (Rat) P46002

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Entrez Gene ID: (Human) 5724, (Mouse) 19204, (Rat) 58949

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lipopolysaccharide binding lipopolysaccharide receptor activity G-protein coupled receptor activity platelet activating factor receptor activity phospholipid binding G-protein coupled purinergic nucleotide receptor activity mitogen-activated protein kinase binding signal transducer activity G-protein coupled receptor

cytokine production regulation of transcription from RNA polymerase II promoter chemotaxis inflammatory response immune response G-protein coupled receptor signaling pathway parturition response to symbiotic bacterium positive regulation of phospholipase C activity lipopolysaccharide-mediated signaling pathway positive regulation of tumor necrosis factor production inositol trisphosphate biosynthetic process G-protein coupled purinergic nucleotide receptor signaling pathway positive regulation of neutrophil degranulation transcytosis positive regulation of interleukin-6 biosynthetic process positive regulation of translation negative regulation of blood pressure positive regulation of vasoconstriction positive regulation of vasodilation phosphatidylinositol-mediated signaling positive regulation of smooth muscle cell proliferation interferon-gamma-mediated signaling pathway positive regulation of inositol phosphate biosynthetic process cellular response to gravity cellular response to cAMP cellular response to fatty acid response to dexamethasone positive regulation of voltage-gated chloride channel activity positive regulation of leukocyte tethering or rolling positive regulation of sensory perception of pain positive regulation of transcytosis positive regulation of maternal process involved in parturition positive regulation of gastro-intestinal system smooth muscle contraction cellular response to 2-O-acetyl-1-O-hexadecyl-sn-glycero-3-phosphocholine pattern recognition receptor signaling pathway signal transduction response to lipopolysaccharide