Invitrogen

Pleckstrin Polyclonal Antibody

查看其他14个Pleckstrin抗体

抗体检测数据 (1)

Pleckstrin Antibody in Western Blot (WB)

Pleckstrin Antibody in Western Blot (WB)

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Pleckstrin Antibody (PA5-119730) in WB

Western blot analysis of Pleckstrin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Pleckstrin Polyclonal Antibody (Product # PA5-119730) at 1:1,000 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: U937 cell lysate. Lane 2: Rat spleen tissue lysate. Lane 2: Mouse brain tissue lysate.

Pleckstrin Antibody in Western Blot (WB)

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Pleckstrin Antibody in Western Blot (WB) — Western blot analysis of Pleckstrin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Pleckstrin Polyclonal Antibody (Product # PA5-119730) at 1:1,000 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: U937 cell lysate. Lane 2: Rat spleen tissue lysate. Lane 2: Mouse brain tissue lysate.

产品信息

PA5-119730

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide within human Pleckstrin aa 300-350

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

TBS, pH 7.4, with 50% glycerol, 0.2% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2913303

产品详细信息

Positive Control: U937 cell lysate, rat spleen tissue lysate, mouse brain tissue lysate.

Subcellular Location: Cytosol, extracellular region, plasma membrane, ruffle membrane, cytoplasm, membrane.

靶标信息

Major protein kinase C substrate of platelets.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: FLJ27168; p47; Platelet 47 kDa protein; Pleckstrin

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基因别名: 2010300B13Rik; P47; PLEK

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UniProt ID: (Human) P08567, (Mouse) Q9JHK5, (Rat) Q4KM33

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Entrez Gene ID: (Human) 5341, (Mouse) 56193, (Rat) 364206

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protein kinase C binding protein binding protein homodimerization activity phosphatidylinositol-3,4-bisphosphate binding cytoskeletal protein

hematopoietic progenitor cell differentiation platelet degranulation vesicle docking involved in exocytosis integrin-mediated signaling pathway positive regulation of platelet activation negative regulation of inositol phosphate biosynthetic process positive regulation of inositol-polyphosphate 5-phosphatase activity cell projection organization positive regulation of actin filament depolymerization phospholipase C-inhibiting G-protein coupled receptor signaling pathway cortical actin cytoskeleton organization ruffle organization actin cytoskeleton reorganization positive regulation of actin filament bundle assembly positive regulation of integrin activation negative regulation of G-protein coupled receptor protein signaling pathway phosphatidylinositol metabolic process negative regulation of calcium-mediated signaling regulation of cell diameter thrombin receptor signaling pathway platelet aggregation protein kinase C signaling protein secretion by platelet intracellular signal transduction