Invitrogen

Pleckstrin Polyclonal Antibody

查看其他14个Pleckstrin抗体

抗体检测数据 (5)

Pleckstrin Antibody in Immunocytochemistry (ICC/IF)

Pleckstrin Antibody in Immunocytochemistry (ICC/IF)

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Pleckstrin Antibody (PA5-18225) in ICC/IF

Immunocytochemistry analysis of Pleckstrin using Pleckstrin Polyclonal Antibody (Product # PA5-18225) in paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic/membrane and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed b... View More

Pleckstrin Antibody in Immunocytochemistry (ICC/IF)

Pleckstrin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Pleckstrin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Pleckstrin Antibody in Western Blot (WB)

Pleckstrin Antibody in Flow Cytometry (Flow)

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Pleckstrin Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of Pleckstrin using Pleckstrin Polyclonal Antibody (Product # PA5-18225) in paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic/membrane and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Pleckstrin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Pleckstrin in human vessel, platelets. Samples were incubated with Pleckstrin polyclonal antibody (Product # PA5-18225) using a dilution of 75 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Pleckstrin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Pleckstrin in human spleen. Samples were incubated with Pleckstrin polyclonal antibody (Product # PA5-18225) using a dilution of 75 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Pleckstrin Antibody in Western Blot (WB) — Western blot analysis of Pleckstrin using Pleckstrin Polyclonal Antibody (Product # PA5-18225) (0.01 µg/mL) in staining of Human Parathyroid lysate (A) and negative control HepG2 (B) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

Pleckstrin Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of Pleckstrin in HeLa cells using a polyclonal antibody (Product #PA5-18225). HeLa cells (blue line) were paraformaldehyde fixed and permeabilized with 0.5% Triton. The primary antibody was incubated for one hour (10 µg/mL) followed by an Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by an Alexa Fluor 488 secondary antibody.

产品信息

PA5-18225

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.01-0.3 µg/mL

-

免疫组化（石蜡） (IHC (P))

3.75 µg/mL

-

免疫细胞化学 (ICC/IF)

10 µg/mL

-

流式细胞分析 (Flow)

10 µg/mL

-

产品规格

种属反应

Human

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide sequence (EWIKAIQMASRTGK) corresponding to the C-terminus amino acids of PLEK (aa 341-350).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

TBS, pH 7.3, with 0.5% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_10984936

产品详细信息

This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.

靶标信息

Major protein kinase C substrate of platelets.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: FLJ27168; p47; Platelet 47 kDa protein; Pleckstrin

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基因别名: P47; PLEK

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UniProt ID: (Human) P08567

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Entrez Gene ID: (Human) 5341

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protein kinase C binding protein binding protein homodimerization activity phosphatidylinositol-3,4-bisphosphate binding

hematopoietic progenitor cell differentiation platelet degranulation vesicle docking involved in exocytosis integrin-mediated signaling pathway positive regulation of platelet activation negative regulation of inositol phosphate biosynthetic process positive regulation of inositol-polyphosphate 5-phosphatase activity cell projection organization positive regulation of actin filament depolymerization phospholipase C-inhibiting G-protein coupled receptor signaling pathway cortical actin cytoskeleton organization ruffle organization actin cytoskeleton reorganization positive regulation of actin filament bundle assembly positive regulation of integrin activation negative regulation of G-protein coupled receptor protein signaling pathway phosphatidylinositol metabolic process negative regulation of calcium-mediated signaling regulation of cell diameter thrombin receptor signaling pathway platelet aggregation protein kinase C signaling protein secretion by platelet