Invitrogen

Pleckstrin Polyclonal Antibody

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抗体检测数据 (1)

Pleckstrin Antibody in Western Blot (WB)

Pleckstrin Antibody in Western Blot (WB)

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Pleckstrin Antibody (PA5-92885) in WB

Western blot analysis of Pleckstrin in extracts of various cell lines. Samples were incubated with Pleckstrin Polyclonal antibody (Product # PA5-92885) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Pleckstrin Antibody in Western Blot (WB)

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Pleckstrin Antibody in Western Blot (WB) — Western blot analysis of Pleckstrin in extracts of various cell lines. Samples were incubated with Pleckstrin Polyclonal antibody (Product # PA5-92885) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

产品信息

PA5-92885

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

酶联免疫吸附实验 (ELISA)

1 µg/mL

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human PLEK (NP_0026552)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2.23 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2806532

产品详细信息

Immunogen sequence: MEPKRIREGY LVKKGSVFNT WKPMWVVLLE DGIEFYKKKS DNSPKGMIPL KGSTLTSPCQ DFGKRMFVFK ITTTKQQDHF FQAAFLEERD AWVRDIKKAI KCIEGGQKFA RKSTRRSIRL PETIDLGALY LSMKDTEKGI KELNLEKDKK IFNHCFTGNC VIDWLVSNQS VRNRQEGLMI ASSLLNEGYL QPAGDMSKSA VDGTAENPFL DNPDAFYYFP DSGFFCEENS SDDDVILKEE FRGVIIKQGC LLKQGHRRKN WKVRKFILRE DPAYLHYYDP AGAEDPLGAI HLRGCVVTSV ESNSNGRKSE EENLFEIITA DEVHYFLQAA TPKERTEWIR AIQMASRTGK; Positive Samples: THP-1, U-937, Mouse lung, Mouse spleen, Rat spleen

靶标信息

Major protein kinase C substrate of platelets.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: FLJ27168; p47; Platelet 47 kDa protein; Pleckstrin

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基因别名: 2010300B13Rik; P47; PLEK

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UniProt ID: (Human) P08567, (Mouse) Q9JHK5, (Rat) Q4KM33

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Entrez Gene ID: (Human) 5341, (Mouse) 56193, (Rat) 364206

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protein kinase C binding protein binding protein homodimerization activity phosphatidylinositol-3,4-bisphosphate binding cytoskeletal protein

hematopoietic progenitor cell differentiation platelet degranulation vesicle docking involved in exocytosis integrin-mediated signaling pathway positive regulation of platelet activation negative regulation of inositol phosphate biosynthetic process positive regulation of inositol-polyphosphate 5-phosphatase activity cell projection organization positive regulation of actin filament depolymerization phospholipase C-inhibiting G-protein coupled receptor signaling pathway cortical actin cytoskeleton organization ruffle organization actin cytoskeleton reorganization positive regulation of actin filament bundle assembly positive regulation of integrin activation negative regulation of G-protein coupled receptor protein signaling pathway phosphatidylinositol metabolic process negative regulation of calcium-mediated signaling regulation of cell diameter thrombin receptor signaling pathway platelet aggregation protein kinase C signaling protein secretion by platelet intracellular signal transduction