Invitrogen

Podoplanin Monoclonal Antibody (8.1.1)

Podoplanin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Podoplanin Antibody (MA5-16113) in IHC (P)

Immunohistochemical analysis of Podoplanin in mouse kidney. Samples were incubated in Podoplanin monoclonal antibody (Product # MA5-16113). Antibody labeling (Green); nuclei were counterstained with DAPI (Blue).

Podoplanin Antibody in Western Blot (WB)

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产品信息

MA5-16113

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:2,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:500

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免疫组化（冰冻） (IHC (F))

Assay-Dependent

免疫细胞化学 (ICC/IF)

1-5 µg/mL

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流式细胞分析 (Flow)

1:400

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免疫沉淀 (IP)

1:10-1:500

免疫电镜技术 (IM)

Assay-Dependent

产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Syrian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

8.1.1

抗原

Murine thymic stromal cell lines

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_11152879

产品详细信息

This antibody does not react with human samples.

靶标信息

Podoplanin (PDPN, T1A, gp38, Aggrus) is a mucin type-1 glycoprotein with a 40-43 kDa molecular weight. Podoplanin is expressed in many tumors and normal cells, especially lymphatic epithelial cells and follicular DCs. Podoplanin localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. Podoplanin appears to serve as a ligand for CLEC-2 and expression is positively correlated with tumors expressing greater invasive and metastatic potential. Podoplanin is directly involved in cell migration, aids metastases formation and tumor cell invasion of tissue. Further, it has also been determined that podoplanin expression is induced through Src activation of Cas and, recently, there are indications of podoplanin presence on Th17 cells. Initial studies below indicate the presence of podoplanin on a small but distinct population of CD4+ cells from human PBMC. Dysfunction of the podoplanin protein has been linked to diseases such as lymphangioma and subglottis neoplasm. Podoplanin has also been proposed as a marker for lung injury due to the physiological function of the protein.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Deletion of the mRNA stability factor ELAVL1 (HuR) in pancreatic cancer cells disrupts the tumor microenvironment integrity.

NAR cancer

McCarthy GA,Di Niro R,Finan JM,Jain A,Guo Y,Wyatt CR,Guimaraes AR,Waugh TA,Keith D,Morgan TK,Sears RC,Brody JR

MA5-16113 was used in Immunohistochemistry-immunofluorescence to identify a novel role of tumor-intrinsic human antigen R in its ability to modify the surrounding tumor microenvironment and regulate platelet-derived growth factor AA (PDGFAA).

Thu Jun 01 00:00:00 UTC 2023

EHMT2 methyltransferase governs cell identity in the lung and is required for KRAS ^G12D tumor development and propagation.

eLife

Pribluda A,Daemen A,Lima AN,Wang X,Hafner M,Poon C,Modrusan Z,Katakam AK,Foreman O,Eastham J,Hung J,Haley B,Garcia JT,Jackson EL,Junttila MR

MA5-16113 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunomicroscopy, Immunoprecipitation, Western Blot to reveal EHMT2 as a critical regulator of Wnt signaling, implicating Ehmt2 as a potential target in lung cancer and other AT2-mediated lung pathologies.

Fri Aug 19 00:00:00 UTC 2022

Prkg2 regulates alveolar type 2-mediated re-alveolarization.

Stem cell research & therapy

Zhang M,Ali G,Komatsu S,Zhao R,Ji HL

MA5-16113 was used in Immunocytochemistry to demonstrate that Prkg2 may regulate the lineage of AT2 cells, which is afected by endotoxins and the interactive PKA signaling pathway.

Mon Mar 21 00:00:00 UTC 2022

Prkg2 regulates alveolar type 2-mediated re-alveolarization.

Stem cell research & therapy

Zhang M,Ali G,Komatsu S,Zhao R,Ji HL

MA5-16113 was used in Immunocytochemistry to demonstrate that Prkg2 may regulate the lineage of AT2 cells, which is afected by endotoxins and the interactive PKA signaling pathway.

Mon Mar 21 00:00:00 UTC 2022

Liver lymphatic drainage patterns follow segmental anatomy in a murine model.

Scientific reports

Frenkel NC,Poghosyan S,Verheem A,Padera TP,Rinkes IHMB,Kranenburg O,Hagendoorn J

MA5-16113 was used in Immunohistochemistry to provide evidence that liver lymphatic drainage patterns follow segmental anatomy.

Fri Dec 11 00:00:00 UTC 2020

Liver lymphatic drainage patterns follow segmental anatomy in a murine model.

Scientific reports

Frenkel NC,Poghosyan S,Verheem A,Padera TP,Rinkes IHMB,Kranenburg O,Hagendoorn J

MA5-16113 was used in Immunohistochemistry to provide evidence that liver lymphatic drainage patterns follow segmental anatomy.

Fri Dec 11 00:00:00 UTC 2020

Mice with a deficiency in CLEC-2 are protected against deep vein thrombosis.

Blood

Payne H,Ponomaryov T,Watson SP,Brill A

MA516113 was used in blocking or activating experiment, immunohistochemistry, and western blot to find mice with general inducible deletion of platelet C-type lectin-like receptor 2 or platelet-specific deficiency in CLEC-2 are protected against deep vein thrombosis

Thu Apr 06 00:00:00 UTC 2017

Altered Dermal Fibroblasts in Systemic Sclerosis Display Podoplanin and CD90.

The American journal of pathology

Nazari B,Rice LM,Stifano G,Barron AM,Wang YM,Korndorf T,Lee J,Bhawan J,Lafyatis R,Browning JL

MA516113 was used in immunohistochemistry - paraffin section to characterize alterations in fibroblast populations in the skin of patients with systemic sclerosis

Sat Oct 01 00:00:00 UTC 2016

Effector lymphocyte-induced lymph node-like vasculature enables naive T-cell entry into tumours and enhanced anti-tumour immunity.

Nature communications

Peske JD,Thompson ED,Gemta L,Baylis RA,Fu YX,Engelhard VH

MA516113 was used in flow cytometry and immunohistochemistry to find that lymph node-like vasculature in melanoma and lung carcinoma murine models is both a consequence of and key contributor to anti-tumor immunity

Wed May 13 00:00:00 UTC 2015

Lymph node stromal cells constrain immunity via MHC class II self-antigen presentation.

eLife

Baptista AP,Roozendaal R,Reijmers RM,Koning JJ,Unger WW,Greuter M,Keuning ED,Molenaar R,Goverse G,Sneeboer MM,den Haan JM,Boes M,Mebius RE

MA5-16113 was used in flow cytometry to examine the role of lymph node stromal cells in immune responses

Wed Nov 19 00:00:00 UTC 2014

生物信息学

蛋白别名: aggrus; CTA-520D8.1; E11; Glycoprotein 38; Gp38; OTS-8; OTTHUMP00000009640; OTTHUMP00000044504; PA2.26 antigen; Podoplanin; RP23-348F1.2; Transmembrane glycoprotein E11

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基因别名: Gp38; OTS-8; Ots8; Pdpn; RANDAM-2; T1-alpha; T1a; T1alpha

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UniProt ID: (Mouse) Q62011

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Entrez Gene ID: (Mouse) 14726

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功能

water transmembrane transporter activity folic acid transporter activity amino acid transmembrane transporter activity water channel activity

参与通路