Invitrogen

Podoplanin Monoclonal Antibody (NZ-1.3), eBioscience™

Podoplanin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Podoplanin Antibody (14-9381-82) in IHC (P)

Immunohistochemical analysis of Podoplanin was performed using formalin-fixed paraffin-embedded human lung squamous cell carcinoma tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 degree ... View More

Podoplanin Antibody in Western Blot (WB)

Podoplanin Antibody in Immunocytochemistry (ICC/IF)

Podoplanin Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

Podoplanin Antibody in Flow Cytometry (Flow)

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产品信息

14-9381-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化 (IHC)

查看5篇已发表文章 5篇已发表文章

免疫组化（石蜡） (IHC (P))

5 µg/mL

免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

查看9篇已发表文章 9篇已发表文章

免疫沉淀 (IP)

Assay-Dependent

功能检测 (Functional)

查看1篇已发表文章 1篇已发表文章

抑制试剂 (IA)

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产品规格

种属反应

Human, Rat

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

NZ-1.3

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_1603307

产品详细信息

Description: Podoplanin is a 40-kDa type I transmembrane sialomucin-like glycoprotein involved in platelet aggregation and cancer metastasis. This protein is expressed on lymphatic endothelial cells and follicular dendritic cells, as well as on many human cancers, including squamous cell carcinoma, glioblastoma, seminoma, and mesothelioma. Podoplanin expression has been demonstrated to be regulated by the Fos protein of the Activating Protein-1 (AP-1) transcription factor complex. Much of its functionality arises via interaction with its endogenous receptor, C-type lectin-like receptor-2 (CLEC-2).

This antibody has been reported to block the interaction between Podoplanin and CLEC-2.

Applications Reported: This NZ-1.3 antibody has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistology staining of paraffin embedded tissue sections.

Applications Tested: This NZ-1.3 antibody has been tested by immunoblot analysis of U87 cells. This can be used at 1-5 µg per mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Podoplanin (PDPN, T1A, gp38, Aggrus) is a mucin type-1 glycoprotein with a 40-43 kDa molecular weight. Podoplanin is expressed in many tumors and normal cells, especially lymphatic epithelial cells and follicular DCs. Podoplanin localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. Podoplanin appears to serve as a ligand for CLEC-2 and expression is positively correlated with tumors expressing greater invasive and metastatic potential. Podoplanin is directly involved in cell migration, aids metastases formation and tumor cell invasion of tissue. Further, it has also been determined that podoplanin expression is induced through Src activation of Cas and, recently, there are indications of podoplanin presence on Th17 cells. Initial studies below indicate the presence of podoplanin on a small but distinct population of CD4+ cells from human PBMC. Dysfunction of the podoplanin protein has been linked to diseases such as lymphangioma and subglottis neoplasm. Podoplanin has also been proposed as a marker for lung injury due to the physiological function of the protein.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

APOLD1 loss causes endothelial dysfunction involving cell junctions, cytoskeletal architecture, and Weibel-Palade bodies, while disrupting hemostasis.

Haematologica

Stritt S,Nurden P,Nurden AT,Schved JF,Bordet JC,Roux M,Alessi MC,Trégouët DA,Mäkinen T,Giansily-Blaizot M

14-9381-82 was used in Functional assay to identify APOLD1 as an important regulator of vascular homeostasis and raise the need to consider testing of EC function in patients with IBDs without apparent platelet or coagulation defects.

Wed Mar 01 00:00:00 UTC 2023

Drivers of heterogeneity in synovial fibroblasts in rheumatoid arthritis.

Nature immunology

Smith MH,Gao VR,Periyakoil PK,Kochen A,DiCarlo EF,Goodman SM,Norman TM,Donlin LT,Leslie CS,Rudensky AY

14-9381-82 was used in Flow Cytometry to highlight a role for concurrent, spatially distributed cytokine signaling within the inflamed synovium.

Sat Jul 01 00:00:00 UTC 2023

A novel cryopreservation and biobanking strategy to study lymphoid tissue stromal cells in human disease.

European journal of immunology

Brandstadter JD,De Martin A,L¿tge M,Ferreira A,Gaudette BT,Stanossek Y,Wang S,Gonzalez MV,Camiolo E,Wertheim G,Austin B,Allman D,Bagg A,Lim MS,Fajgenbaum DC,Aster JC,Ludewig B,Maillard I

14-9381-82 was used in Immunohistochemistry to demonstrate that cryopreservation can be used to bank lymphoid tissue for the study of LNSCs in human disease.

Fri Sep 01 00:00:00 UTC 2023

PI16⁺ reticular cells in human palatine tonsils govern T cell activity in distinct subepithelial niches.

Nature immunology

De Martin A,Stanossek Y,Lütge M,Cadosch N,Onder L,Cheng HW,Brandstadter JD,Maillard I,Stoeckli SJ,Pikor NB,Ludewig B

14-9381-82 was used in Immunohistochemistry to show that human tonsillar FRCs undergo dynamic reprogramming during life and respond vigorously to inflammatory perturbation in comparison to other stromal cell types.

Sat Jul 01 00:00:00 UTC 2023

Reprogramming bone progenitor identity and potency through control of collagen density and oxygen tension.

iScience

Al Hosni R,Bozec L,Roberts SJ,Cheema U

Published figure using Podoplanin monoclonal antibody (Product # 14-9381-82) in Flow Cytometry

Fri Apr 15 00:00:00 UTC 2022

Dissecting human embryonic skeletal stem cell ontogeny by single-cell transcriptomic and functional analyses.

Cell research

He J,Yan J,Wang J,Zhao L,Xin Q,Zeng Y,Sun Y,Zhang H,Bai Z,Li Z,Ni Y,Gong Y,Li Y,He H,Bian Z,Lan Y,Ma C,Bian L,Zhu H,Liu B,Yue R

Published figure using Podoplanin monoclonal antibody (Product # 14-9381-82) in Flow Cytometry

Thu Jul 01 00:00:00 UTC 2021

Dissecting human embryonic skeletal stem cell ontogeny by single-cell transcriptomic and functional analyses.

Cell research

He J,Yan J,Wang J,Zhao L,Xin Q,Zeng Y,Sun Y,Zhang H,Bai Z,Li Z,Ni Y,Gong Y,Li Y,He H,Bian Z,Lan Y,Ma C,Bian L,Zhu H,Liu B,Yue R

Published figure using Podoplanin monoclonal antibody (Product # 14-9381-82) in Flow Cytometry

Thu Jul 01 00:00:00 UTC 2021

Kaposi's sarcoma-associated herpesvirus vFLIP promotes MEndT to generate hybrid M/E state for tumorigenesis.

PLoS pathogens

Chen W,Ding Y,Liu D,Lu Z,Wang Y,Yuan Y

Published figure using Podoplanin monoclonal antibody (Product # 14-9381-82) in Flow Cytometry

Wed Dec 01 00:00:00 UTC 2021

Directed induction of alveolar type I cells derived from pluripotent stem cells via Wnt signaling inhibition.

Stem cells (Dayton, Ohio)

Kanagaki S,Ikeo S,Suezawa T,Yamamoto Y,Seki M,Hirai T,Hagiwara M,Suzuki Y,Gotoh S

Published figure using Podoplanin monoclonal antibody (Product # 14-9381-82) in Immunocytochemistry

Mon Feb 01 00:00:00 UTC 2021

Directed induction of alveolar type I cells derived from pluripotent stem cells via Wnt signaling inhibition.

Stem cells (Dayton, Ohio)

Kanagaki S,Ikeo S,Suezawa T,Yamamoto Y,Seki M,Hirai T,Hagiwara M,Suzuki Y,Gotoh S

Published figure using Podoplanin monoclonal antibody (Product # 14-9381-82) in Immunocytochemistry

Mon Feb 01 00:00:00 UTC 2021

Distinctive Properties of Endothelial Cells from Tumor and Normal Tissue in Human Breast Cancer.

International journal of molecular sciences

Wilkus K,Brodaczewska K,Kajdasz A,Kieda C

Published figure using Podoplanin monoclonal antibody (Product # 14-9381-82) in Flow Cytometry

Tue Aug 17 00:00:00 UTC 2021

Phenotypic and functional characterization of synovial fluid-derived fibroblast-like synoviocytes in rheumatoid arthritis.

Scientific reports

Køster D,Egedal JH,Lomholt S,Hvid M,Jakobsen MR,Müller-Ladner U,Eibel H,Deleuran B,Kragstrup TW,Neumann E,Nielsen MA

Published figure using Podoplanin monoclonal antibody (Product # 14-9381-82) in Flow Cytometry

Fri Nov 12 00:00:00 UTC 2021

T helper cells in synovial fluid of patients with rheumatoid arthritis primarily have a Th1 and a CXCR3⁺Th2 phenotype.

Arthritis research & therapy

Aldridge J,Ekwall AH,Mark L,Bergström B,Andersson K,Gjertsson I,Lundell AC,Rudin A

14-9381-82 was used in Flow Cytometry to examined the proportions of Th1, Th2, CXCR3+Th2, Th17, CXCR3+Th17, Th1Th17, peripheral T helper (TPh) and T follicular helper (TFh) cells in paired SF and blood, as well as the phenotype of TPh and TFh cells in RA SF.

Fri Oct 16 00:00:00 UTC 2020

Functionally distinct disease-associated fibroblast subsets in rheumatoid arthritis.

Nature communications

Mizoguchi F,Slowikowski K,Wei K,Marshall JL,Rao DA,Chang SK,Nguyen HN,Noss EH,Turner JD,Earp BE,Blazar PE,Wright J,Simmons BP,Donlin LT,Kalliolias GD,Goodman SM,Bykerk VP,Ivashkiv LB,Lederer JA,Hacohen N,Nigrovic PA,Filer A,Buckley CD,Raychaudhuri S,Brenner MB

14-9381 was used in Immunohistochemistry-immunofluorescence to identify seven fibroblast subsets with distinct surface protein phenotypes, and characterise them into three subsets by integrating transcriptomic data.

Fri Feb 23 00:00:00 UTC 2018

Human Extravillous Trophoblasts Penetrate Decidual Veins and Lymphatics before Remodeling Spiral Arteries during Early Pregnancy.

PloS one

He N,van Iperen L,de Jong D,Szuhai K,Helmerhorst FM,van der Westerlaken LA,Chuva de Sousa Lopes SM

Published figure using Podoplanin monoclonal antibody (Product # 14-9381-82) in Immunofluorescence

Thu Aug 03 00:00:00 UTC 2017

Role of podoplanin in the high interleukin-17A secretion resulting from interactions between activated lymphocytes and psoriatic skin-derived mesenchymal cells.

Clinical and experimental immunology

Noack M,Ndongo-Thiam N,Miossec P

14-9381-82 was used in Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Flow Cytometry to identify a mechanism which leads to a high interleukin (IL)-17 secretion.

Sat Oct 01 00:00:00 UTC 2016

CCL7 contributes to the TNF-alpha-dependent inflammation of lesional psoriatic skin.

Experimental dermatology

Brunner PM,Glitzner E,Reininger B,Klein I,Stary G,Mildner M,Uhrin P,Sibilia M,Stingl G

14938182 was used in immunohistochemistry to propose that CCL7 is a driver of TNF-alpha-dependent T cell-mediated inflammation in lesional psoriatic skin

Wed Jul 01 00:00:00 UTC 2015

CLEC-2 and Syk in the megakaryocytic/platelet lineage are essential for development.

Blood

Finney BA,Schweighoffer E,Navarro-Núñez L,Bénézech C,Barone F,Hughes CE,Langan SA,Lowe KL,Pollitt AY,Mourao-Sa D,Sheardown S,Nash GB,Smithers N,Reis e Sousa C,Tybulewicz VL,Watson SP

14-9381-82 was used in Inhibition experiments to study the relationship between the C-type lectin receptor, CLEC-2, and the tyrosine kinase Syk on multiple developmental processes in mice.

Thu Feb 16 00:00:00 UTC 2012

生物信息学

蛋白别名: Aggrus; CTA-520D8.1; E11 antigen epitope; epithelial cell surface transmembrane protein antigen; Glycoprotein 36; glycoprotein 38; glycoprotein, 36-KD; Gp36; hT1alpha-1; hT1alpha-2; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2); OTTHUMP00000009640; OTTHUMP00000044504; PA2.26 antigen; PDPN; Podoplanin; pulmonary type I alveolar epithelial cell transmembrane differentiation marker; RP23-348F1.2; RTI140; T1-alpha; T1A; Type I cell 40 kDa protein

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基因别名: AGGRUS; E11; GP36; Gp38; GP40; HT1A-1; OTS-8; OTS8; PA2.26; PDPN; PSEC0003; PSEC0025; RTI40; T1-alpha; T1A; T1A-2; T1A2; TI1A

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UniProt ID: (Human) Q86YL7, (Rat) Q64294

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Entrez Gene ID: (Human) 10630, (Rat) 54320

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功能

water transmembrane transporter activity folic acid transporter activity amino acid transmembrane transporter activity water channel activity molecular_function

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