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Podoplanin Chimeric Antibody (MA5-48089) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of Podoplanin in HEK293 cells using Podoplanin Chimeric Monoclonal Antibody (Product # MA5-48089). Cells were transfected with Podoplanin (MAP tag) expressing plasmid (A) and non-transfected HEK293 cells (B), permeabilized with 0.15% Triton stained with Podoplanin Chimeric Monoclonal Antibody at a dilution of 1:200 for 1h followed by Alexa Fluor 488 secondary ... View More
产品信息
MA5-48089
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
PMab-1
抗原
This antibody was raised by immunising rats with 14-residue synthetic peptide mpp3851, which corresponds to amino acids 38-51 (GDGMVPPGIEDKIT) of the platelet-aggregation-stimulating (PLAG) domain of mouse podoplanin.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.02% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_3074532
产品详细信息
Specificity: This antibody is specific for amino acids 38-51 (GDGMVPPGIEDKIT) of the platelet-aggregation-stimulating (PLAG) domain of mouse podoplanin (PDPN). This antibody, therefore, possesses high affinity and specificity for the MAP epitope tag (GDGMVPPGIEDK), which is derived from the PLAG domain of murine podoplanin.
Preparation: This antibody was raised by immunising rats with 14-residue synthetic peptide mpp3851, which corresponds to amino acids 38-51 (GDGMVPPGIEDKIT) of the platelet-aggregation-stimulating (PLAG) domain of mouse podoplanin. Spleen cells were then harvested, and fused to P3U1 cells to generate stable hybridomas.
靶标信息
Podoplanin (PDPN, T1A, gp38, Aggrus) is a mucin type-1 glycoprotein with a 40-43 kDa molecular weight. Podoplanin is expressed in many tumors and normal cells, especially lymphatic epithelial cells and follicular DCs. Podoplanin localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. Podoplanin appears to serve as a ligand for CLEC-2 and expression is positively correlated with tumors expressing greater invasive and metastatic potential. Podoplanin is directly involved in cell migration, aids metastases formation and tumor cell invasion of tissue. Further, it has also been determined that podoplanin expression is induced through Src activation of Cas and, recently, there are indications of podoplanin presence on Th17 cells. Initial studies below indicate the presence of podoplanin on a small but distinct population of CD4+ cells from human PBMC. Dysfunction of the podoplanin protein has been linked to diseases such as lymphangioma and subglottis neoplasm. Podoplanin has also been proposed as a marker for lung injury due to the physiological function of the protein.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: aggrus; CTA-520D8.1; E11; Glycoprotein 38; Gp38; OTS-8; OTTHUMP00000009640; OTTHUMP00000044504; PA2.26 antigen; Podoplanin; RP23-348F1.2; Transmembrane glycoprotein E11
基因别名: Gp38; OTS-8; Ots8; Pdpn; RANDAM-2; T1-alpha; T1a; T1alpha
UniProt ID: (Mouse) Q62011
Entrez Gene ID: (Mouse) 14726
cell morphogenesis
lymphangiogenesis
prostaglandin metabolic process
water transport
amino acid transport
movement of cell or subcellular component
cell adhesion
signal transduction
multicellular organism development
cell proliferation
regulation of cell shape
positive regulation of platelet activation
folic acid transport
single organismal cell-cell adhesion
lung development
positive regulation of cell migration
tube morphogenesis
lung alveolus development
positive regulation of cellular component movement
visceral serous pericardium development
regulation of G1/S transition of mitotic cell cycle
