Invitrogen

Pre-Pro-Vasopressin Polyclonal Antibody

产品信息

PA5-143640

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1:1,000

免疫细胞化学 (ICC/IF)

1:1,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to key amino acid residues surrounding the cleavage sites for the human vasopressin precursor peptide, conjugated to keyhole limpet hemocyanin (KLH).

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

10mM HEPES, pH 7.5, with 100µg/mL BSA, 50% glycerol, 150mM NaCl

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2942868

产品详细信息

Specific for locally produced levels of the ~ 20 kDa uncleaved pre-pro-vasopressin protein. Immunolabeling of the uncleaved pre-pro-vasopressin protein band is completely blocked by preadsorption of the antibody with the peptide used as antigen.

靶标信息

Vasopressin is a nine amino-acid peptide hormone that plays a key role in water and blood pressure homeostasis (Qureshi S., et al 2014). Vasopressin is the end-product of a highly processed 164 amino acid pre-pro-peptide. Processing of the vasopressin pre-pro-peptide results in three distinct peptides with a 1:1:1 ratio: vasopressin, neurophysin II, and copeptin (Arroyo J.P. et al, 2021). Vasopressin is the biologically active hormone, neurophysin II is a carrier molecule for vasopressin, and copeptin is the c-terminal glycosylated end-product. Vasopressin has been thought to be primarily made in the brain, and the sole source of vasopressin stimulating vasopressin V2 receptors in the kidney until recent studies (Arroyo JP, et al 2022). This antibody specially detects uncleaved pre-pro-vasopressin, accurately identifying locally produced vasopressin versus peripheral uptake of hypothalamic vasopressin or related peptides (Arroyo J.P. et al, 2022). This key research tool aided the Arroyo group in discovering immunoreactive vasopressin outside of the brain by recognizing localized vasopressin in human and mouse kidney, specifically in the distal nephron.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: antidiuretic hormone; arginine vasopressin (Diabetes insipidus), same as Di (conflicting physical mapping); ARVP; AVP-NPII; copeptin; neurohypophyseal; prepro-arginine-vasopressin-neurophysin II; prepro-AVP-NP II; prepropressophysin; preprovasopressin-neurophysin; Vasopressin-neurophysin 2-copeptin; vasopressin-neurophysin II-copeptin; vasopressin-neurophysin prepropeptide; VP

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基因别名: ADH; ARVP; AVP; AVP-NPII; AVRP; DI; Vas; VP; Vsp

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UniProt ID: (Human) P01185, (Mouse) P35455, (Rat) P01186

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Entrez Gene ID: (Human) 551, (Mouse) 11998, (Rat) 24221

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