Invitrogen

Presenilin 1 Monoclonal Antibody (APS 11)

1 篇参考文献
查看其他20个Presenilin 1抗体
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说明书
实验方案
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产品信息

MA1-751

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:100-1:1,000
-

免疫组化（石蜡） (IHC (P))

0.75 µg/mL
-

免疫组织化学（多聚甲醛固定） (IHC (PFA))

Assay-dependent
-

免疫细胞化学 (ICC/IF)

Assay-dependent
-

酶联免疫吸附实验 (ELISA)

4.5 µg/mL
-

免疫沉淀 (IP)

Assay-dependent
-

其他PubMed (MISC)

-
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产品规格

种属反应

Human, Mouse, Non-human primate, Rat

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

APS 11

抗原

Synthetic peptide corresponding to residues C H(21) L S N T V R S Q N D N R E(34) of human PS1.
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_325074

产品详细信息

MA1-751 detects presenilin 1 protein (PS1) from mouse, rat, human, and nonhuman primate samples. No cross-reactivity is seen with presenilin 2.

MA1-751 has successfully been used immunofluorescence, immunocytochemistry, immunohistochemistry, Western blot , and ELISA procedures. By Western blot, this antibody detects an ~28 kDa protein representing PS1 N-terminus cleavage product from ST15 cell lysate transfected with human PS1. In formalin-fixed, paraffin embedded sections of human brain, this antibody showed strong staining of both the plaque core and dystrophic neurites.

The MA1-751 immunogen is a synthetic peptide corresponding to residues C H(21) L S N T V R S Q N D N R E(34) of human PS1.

靶标信息

Presenilin1 was initially identified a marker of susceptibility to early-onset Alzheimer's disease. In addition to PEN2, nicastrin and APH-1, Presenilin1 forms the -gamma-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimer's disease. Like the tumor necrosis factor-alpha-converting enzyme (TACE) and the beta-site cleavage enzyme (BACE) protease families, -gamma-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the alpha and -gamma cleavage site) or the toxic Abeta amyloid peptide (from the beta and -gamma cleavage site). It is thought that accumulation of the Abeta peptide is the precursor to Alzheimer's disease. Multiple isoforms of presenilin1 are known to exist.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Alzheimer Disease 3; Minilin; Presenilin-1; Presenilin-1 CTF subunit; Presenilin-1 CTF12; Presenilin-1 NTF subunit; presenilin1; Protein S182; PS-1; PS1-CTF12; S182 protein; Senilin 1

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基因别名: AD3; Ad3h; FAD; PS-1; PS1; PSEN1; PSNL1; S182

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UniProt ID: (Human) P49768, (Rat) P97887

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Entrez Gene ID: (Human) 5663, (Rat) 29192, (Mouse) 19164

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功能
endopeptidase activity aspartic-type endopeptidase activity calcium channel activity protein binding beta-catenin binding PDZ domain binding cadherin binding peptidase activity hydrolase activity aspartic endopeptidase activity, intramembrane cleaving
参与通路
autophagosome assembly negative regulation of transcription from RNA polymerase II promoter activation of MAPKK activity blood vessel development cell fate specification somitogenesis neuron migration positive regulation of receptor recycling heart looping hematopoietic progenitor cell differentiation T cell activation involved in immune response neural retina development protein glycosylation membrane protein ectodomain proteolysis mitochondrial transport cellular response to DNA damage stimulus response to oxidative stress negative regulation of epidermal growth factor-activated receptor activity Notch signaling pathway Notch receptor processing memory post-embryonic development protein transport L-glutamate transport choline transport synaptic vesicle targeting single organismal cell-cell adhesion protein processing cerebral cortex cell migration Cajal-Retzius cell differentiation dorsal/ventral neural tube patterning embryonic limb morphogenesis positive regulation of proteasomal ubiquitin-dependent protein catabolic process endoplasmic reticulum calcium ion homeostasis beta-amyloid formation intracellular signal transduction regulation of phosphorylation amyloid precursor protein catabolic process myeloid dendritic cell differentiation positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of catalytic activity regulation of protein binding positive regulation of MAP kinase activity negative regulation of neuron apoptotic process skin morphogenesis positive regulation of transcription, DNA-templated regulation of synaptic plasticity thymus development neuron development skeletal system morphogenesis brain morphogenesis epithelial cell proliferation negative regulation of axonogenesis positive regulation of coagulation T cell receptor signaling pathway neuron apoptotic process negative regulation of ubiquitin-protein transferase activity smooth endoplasmic reticulum calcium ion homeostasis regulation of synaptic transmission, glutamatergic canonical Wnt signaling pathway regulation of resting membrane potential positive regulation of dendritic spine development calcium ion transmembrane transport negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of apoptotic signaling pathway negative regulation of protein phosphorylation positive regulation of protein phosphorylation myeloid leukocyte differentiation negative regulation of protein kinase activity proteolysis calcium ion transport cellular calcium ion homeostasis autophagy regulation of epidermal growth factor-activated receptor activity brain development heart development learning or memory cerebral cortex development neurogenesis neuron differentiation forebrain development segmentation locomotion eye photoreceptor cell development cellular protein metabolic process positive regulation of protein kinase activity beta-amyloid metabolic process protein maturation apoptotic process cell adhesion embryo development anagen
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