Invitrogen

Profilin 1 Polyclonal Antibody

Profilin 1 Antibody in Immunocytochemistry (ICC/IF)

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Profilin 1 Antibody (PA5-17444) in ICC/IF

Immunofluorescent analysis of Profilin-1 in C2C12 cells using a Profilin-1 polyclonal antibody (Product # PA5-17444) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.

Profilin 1 Antibody in Western Blot (WB)

Profilin 1 Antibody in Flow Cytometry (Flow)

Profilin 1 Antibody in Mass Spectrometry (MS)

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产品信息

PA5-17444

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看3篇已发表文章 3篇已发表文章

免疫细胞化学 (ICC/IF)

1:50

流式细胞分析 (Flow)

1:100

Mass Spectrometry (MS)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Bovine, Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to human profilin-1

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

78 µg/mL

纯化类型

Antigen affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10984035

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role in both signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Profilin I appears to be highly expressed in most tissues except for skeletal muscle. Profilin II is predominantly expressed in brain and at lower levels in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Detergent-insoluble PFN1 inoculation expedites disease onset and progression in PFN1 transgenic rats.

Frontiers in neuroscience

Cui S,Zhang T,Xiong X,Zhao J,Cao Q,Zhou H,Xia XG

PA5-17444 was used in Western Blot to shed light on a novel mechanism that underscores the prion-like characteristics of pathogenic PFN1 in driving neurodegeneration in the context of PFN1-related ALS.

Fri Sep 27 00:00:00 UTC 2024

Detergent-insoluble PFN1 inoculation expedites disease onset and progression in PFN1 transgenic rats.

Frontiers in neuroscience

Cui S,Zhang T,Xiong X,Zhao J,Cao Q,Zhou H,Xia XG

Fri Sep 27 00:00:00 UTC 2024

Detergent-insoluble PFN1 inoculation expedites disease onset and progression in PFN1 transgenic rats.

Frontiers in neuroscience

Cui S,Zhang T,Xiong X,Zhao J,Cao Q,Zhou H,Xia XG

Fri Sep 27 00:00:00 UTC 2024

Profilin 1 delivery tunes cytoskeletal dynamics toward CNS axon regeneration.

The Journal of clinical investigation

Pinto-Costa R,Sousa SC,Leite SC,Nogueira-Rodrigues J,Ferreira da Silva T,Machado D,Marques J,Costa AC,Liz MA,Bartolini F,Brites P,Costell M,Fässler R,Sousa MM

PA5-17444 was used in Western Blotting to identify Pfn1 as an important regulator of axonal regeneration and suggest that AAV-mediated delivery of constitutively active Pfn1, together with the identification of modulators of Pfn1 activity, should be considered to treat the injured nervous system.

Wed Apr 01 00:00:00 UTC 2020

The Loss of Profilin 1 Causes Early Onset Paget's Disease of Bone.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Scotto di Carlo F,Pazzaglia L,Esposito T,Gianfrancesco F

PA5-17444 was used in Western Blotting to investigate an extended pedigree with 11 individuals affected by early onset and polyostotic PDB, mainly interesting the appendicular skeleton.