Invitrogen

Profilin 1 Polyclonal Antibody

Profilin 1 Antibody in Immunocytochemistry (ICC/IF)

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Profilin 1 Antibody (PA5-27612) in ICC/IF

Immunofluorescence analysis of Profilin 1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Profilin 1 Polyclonal Antibody (Product # PA5-27612) at 1:100 dilution in 0.1% BSA, incubated at 4 degree ce... View More

Profilin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Profilin 1 Antibody in Western Blot (WB)

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产品信息

PA5-27612

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 1 and 140 of Human Profilin 1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.56 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2545088

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2, mouse spleen, Rat spleen.

Predicted reactivity: Mouse (95%), Rat (95%), Pig (94%), Bovine (93%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role in both signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Profilin I appears to be highly expressed in most tissues except for skeletal muscle. Profilin II is predominantly expressed in brain and at lower levels in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。