Invitrogen

Progesterone Receptor Polyclonal Antibody

Progesterone Receptor Antibody in Immunocytochemistry (ICC/IF)

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Progesterone Receptor Antibody (PA5-68778) in ICC/IF

Immunofluorescence analysis of Progesterone Receptor was performed using 70% confluent log phase T-47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Progesterone Receptor Polyclonal Antibody (Product # PA5-68778) at 1:100 dilution in 0.1% BSA, incub... View More

Progesterone Receptor Antibody in Western Blot (WB)

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产品信息

PA5-68778

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.2-1.0 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

synthetic peptide directed towards the middle region of mouse Progesterone Receptor

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS with 2% sucrose

内含物

0.09% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2689394

产品详细信息

This target displays homology in the following species: Dog: 91%; Guinea Pig: 91%; Mouse: 100%; Rat: 91%

靶标信息

The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.

仅用于科研。不用于诊断过程。未经明确授权不得转售。