Invitrogen

Pyruvate Carboxylase Polyclonal Antibody

Pyruvate Carboxylase Antibody in Immunocytochemistry (ICC/IF)

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Pyruvate Carboxylase Antibody (PA5-120984) in ICC/IF

Immunofluorescence analysis of Pyruvate Carboxylase in U2OS cells. Samples were incubated with Pyruvate Carboxylase Polyclonal antibody (Product # PA5-120984). Blue: DAPI for nuclear staining.

Pyruvate Carboxylase Antibody in Western Blot (WB)

Pyruvate Carboxylase Antibody in Immunoprecipitation (IP)

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产品信息

PA5-120984

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

免疫细胞化学 (ICC/IF)

1:10-1:100

酶联免疫吸附实验 (ELISA)

1 µg/mL

免疫沉淀 (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 879-1178 of human Pyruvate carboxylase (Pyruvate carboxylase (PC)) (NP_0010358061)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.96 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2914556

产品详细信息

Positive test controls include: HepG2, HeLa, MCF7, A-549, U-251MG, Mouse liver, Mouse brain, Rat liver, Rat brain. The target is usually found in the following locations: Mitochondrion matrix.

Immunogen sequence: HSMGLGSKFK EVKKAYVEAN QMLGDLIKVT PSSKIVGDLA QFMVQNGLSR AEAEAQAEEL SFPRSVVEFL QGYIGVPHGG FPEPFRSKVL KDLPRVEGRP GASLPPLDLQ ALEKELVDRH GEEVTPEDVL SAAMYPDVFA HFKDFTATFG PLDSLNTRLF LQGPKIAEEF EVELERGKTL HIKALAVSDL NRAGQRQVFF ELNGQLRSIL VKDTQAMKEM HFHPKALKDV KGQIGAPMPG KVIDIKVVAG AKVAKGQPLC VLSAMKMETV VTSPMEGTVR KVHVTKDMTL EGDDLILEIE

靶标信息

Pyruvate Carboxylase requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency.

仅用于科研。不用于诊断过程。未经明确授权不得转售。