Invitrogen

Pyruvate Carboxylase Polyclonal Antibody

Pyruvate Carboxylase Antibody in Immunocytochemistry (ICC/IF)

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Pyruvate Carboxylase Antibody (PA5-85705) in ICC/IF

Pyruvate Carboxylase Polyclonal Antibody detects Pyruvate Carboxylase protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Pyruvate Carboxylase protein stained by Pyruvate Carboxylase Polyclonal Antibody (Product # PA5-85705) diluted at 1:500. Blue: Hoechst 33342 staining.

Pyruvate Carboxylase Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Pyruvate Carboxylase Antibody in Western Blot (WB)

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产品信息

PA5-85705

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the C-terminus region of human Pyruvate Carboxylase.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

3.08 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2792844

产品详细信息

Keep as concentrated solution.

Predicted reactivity: Rat (100%), Pig (100%), Rhesus Monkey (100%), Bovine (100%).

Positive Control: 293T, A431, HeLa, mouse liver.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Pyruvate Carboxylase requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency.

仅用于科研。不用于诊断过程。未经明确授权不得转售。