Invitrogen

CD27 Monoclonal Antibody (CLB-27/1), Qdot™ 655

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CD27 Antibody (Q10066) in Flow

Qdot® 655 anti-human CD27 conjugate 655/20 band pass filter

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产品信息

Q10066

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

Assay-dependent

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其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

CLB-27/1

抗原

Human CD27.

偶联物

Qdot™ 655 Qdot™ 655 Qdot™ 655

激发/发射光谱

300/654 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

0.05M borate, pH 8.3, with 1M betaine

内含物

0.05% sodium azide

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_11180873

靶标信息

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Differential effects of the second SARS-CoV-2 mRNA vaccine dose on T cell immunity in naive and COVID-19 recovered individuals.

Cell reports

Lozano-Ojalvo D,Camara C,Lopez-Granados E,Nozal P,Del Pino-Molina L,Bravo-Gallego LY,Paz-Artal E,Pion M,Correa-Rocha R,Ortiz A,Lopez-Hoyos M,Iribarren ME,Portoles J,Rojo-Portoles MP,Ojeda G,Cervera I,Gonzalez-Perez M,Bodega-Mayor I,Montes-Casado M,Portoles P,Perez-Olmeda M,Oteo J,Sanchez-Tarjuelo R,Pothula V,Schwarz M,Brahmachary M,Tan AT,Le Bert N,Berin C,Bertoletti A,Guccione E,Ochando J

Q10066 was used in Flow Cytometry to demonstrate that, while the second dose increases both the humoral and cellular immunity in naive individuals, COVID-19 recovered individuals reach their peak of immunity after the first dose.

Tue Aug 24 00:00:00 UTC 2021

Persistence of HIV-1 Env-Specific Plasmablast Lineages in Plasma Cells after Vaccination in Humans.

Cell reports. Medicine

Basu M,Piepenbrink MS,Francois C,Roche F,Zheng B,Spencer DA,Hessell AJ,Fucile CF,Rosenberg AF,Bunce CA,Liesveld J,Keefer MC,Kobie JJ

Q10066 was used in Flow cytometry/Cell sorting to identify a cellular linkage for the development of sustained Env-specific Abs following vaccination in humans.

Tue May 19 00:00:00 UTC 2020

Differentiation into an Effector Memory Phenotype Potentiates HIV-1 Latency Reversal in CD4⁺ T Cells.

Journal of virology

Kulpa DA,Talla A,Brehm JH,Ribeiro SP,Yuan S,Bebin-Blackwell AG,Miller M,Barnard R,Deeks SG,Hazuda D,Chomont N,Sékaly RP

Q10066 was used in Flow Cytometry to support the idea that the effector memory phenotype supports HIV-1 latency reversal in CD4+ T cells.

Sun Dec 15 00:00:00 UTC 2019

CD4+ T Cells Expressing PD-1, TIGIT and LAG-3 Contribute to HIV Persistence during ART.

PLoS pathogens

Fromentin R,Bakeman W,Lawani MB,Khoury G,Hartogensis W,DaFonseca S,Killian M,Epling L,Hoh R,Sinclair E,Hecht FM,Bacchetti P,Deeks SG,Lewin SR,Sékaly RP,Chomont N

Q10066 was used in flow cytometry test if CD4 positive T cells expressing immune checkpoint molecules are enriched in HIV-infected cells in patients receiving antiretroviral therapy

Fri Jul 01 00:00:00 UTC 2016

Increased Steady-State Memory B Cell Subsets Among High-Risk Participants in an HIV Vaccine Trial.

AIDS research and human retroviruses

Keefer MC,Zheng B,Rosenberg AF,Kobie JJ

Q10066 was used in flow cytometry to investigate how differences in B cell populations may contribute to HIV susceptibility

Mon Dec 18 00:00:00 UTC 2017

Humoral Dysregulation Associated with Increased Systemic Inflammation among Injection Heroin Users.

PloS one

Piepenbrink MS,Samuel M,Zheng B,Carter B,Fucile C,Bunce C,Kiebala M,Khan AA,Thakar J,Maggirwar SB,Morse D,Rosenberg AF,Haughey NJ,Valenti W,Keefer MC,Kobie JJ

Q10066 was used in flow cytometry to characterize B cells and antibody repertoires from injection drug users

Mon Jul 31 00:00:00 UTC 2017

Subinfectious hepatitis C virus exposures suppress T cell responses against subsequent acute infection.

Nature medicine

Park SH,Veerapu NS,Shin EC,Biancotto A,McCoy JP,Capone S,Folgori A,Rehermann B

Q10066 was used in flow cytometry to decipher how susequent acute infection is supressed in T cell responses after exposure to subinfectious hepatitis C virus

Sun Dec 01 00:00:00 UTC 2013

Effects of immunosuppression on circulating adeno-associated virus capsid-specific T cells in humans.

Human gene therapy

Parzych EM,Li H,Yin X,Liu Q,Wu TL,Podsakoff GM,High KA,Levine MH,Ertl HC

Q10066 was used in immunocytochemistry to assess the effect of immunosuppression used for organ transplantation on immune responses to adeno-associated virus capsid antigens.

Mon Apr 01 00:00:00 UTC 2013

9G4 autoreactivity is increased in HIV-infected patients and correlates with HIV broadly neutralizing serum activity.

PloS one

Kobie JJ,Alcena DC,Zheng B,Bryk P,Mattiacio JL,Brewer M,Labranche C,Young FM,Dewhurst S,Montefiori DC,Rosenberg AF,Feng C,Jin X,Keefer MC,Sanz I

Q10066 was used in flow cytometry to assess the breakdown of tolerance in HIV patients.

Mon Nov 19 00:00:00 UTC 2012

Capsid-specific T-cell responses to natural infections with adeno-associated viruses in humans differ from those of nonhuman primates.

Molecular therapy : the journal of the American Society of Gene Therapy

Li H,Lasaro MO,Jia B,Lin SW,Haut LH,High KA,Ertl HC

Q10066 was used in flow cytometry to test if rejection of AAV-transduced hepatocytes is due to AAV capsid-specific CD8 positive T cells that become reactivated upon gene transfer.

Tue Nov 01 00:00:00 UTC 2011

生物信息学

蛋白别名: CD27; CD27 antigen; CD27L receptor; LPFS2; sCD27; soluble CD27; T cell activation antigen S152; T-cell activation antigen CD27; T14; TNFSF7; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7

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基因别名: CD27; S152; S152. LPFS2; T14; TNFRSF7; Tp55

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UniProt ID: (Human) P26842

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Entrez Gene ID: (Human) 939

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功能

transmembrane signaling receptor activity tumor necrosis factor-activated receptor activity protein binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process

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