Invitrogen

QKI Polyclonal Antibody

4个已发表图片

8 篇参考文献

查看其他27个QKI抗体

抗体检测数据 (3)
已发表图片 (4)

QKI Antibody in Immunocytochemistry (ICC/IF)

QKI Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 7

QKI Antibody (PA5-87292) in ICC/IF

Immunofluorescence analysis of QKI in MCF-7 cells. Sample was incubated with polyclonal QKI antibody (Product # PA5-87292).

QKI Antibody in Immunocytochemistry (ICC/IF)

QKI Antibody in Western Blot (WB)

QKI Antibody in Western Blot (WB)

QKI Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)

QKI Antibody in Western Blot (WB)

QKI Antibody in Western Blot, Immunoprecipitation (WB, IP)

QKI Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

QKI Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of QKI in MCF-7 cells. Sample was incubated with polyclonal QKI antibody (Product # PA5-87292).

QKI Antibody in Western Blot (WB) — Western blot analysis of QKI in various cell lines. Sample was incubated with QKI polyclonal antibody (Product # PA5-87292).

QKI Antibody in Western Blot (WB) — Western blot analysis of QKI in various cell lines. Sample was incubated with QKI polyclonal antibody (Product # PA5-87292).

QKI Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP) — CircLIMA1-dependent sperm nuclear actin organization along the epididymis. qRT-PCR detection of circLIMA1 expression levels in caput and cauda SPZ (A) or epididymis (B) from WT and CB1 -/- mice. (C) Immunofluorescence analysis of F-actin by phalloidin staining in caput and cauda SPZ from WT and CB1 -/- mice. qRT-PCR detection of circLIMA1 expression levels (D) and Immunofluorescence analysis of F-actin by phalloidin staining (E) in caput SPZ from WT mice in vitro co-incubated with: WT Caput ELF (CTRL group), WT Caput ELF combined with Cytochalasin-D (C8273; Sigma-Aldrich, Milano, Italy) at the concentration of 10 muM (ELF WT+CYTOD); CB1 -/- Caput ELF (ELF CB1 -/-); CB1 -/- Caput ELF combined with Cytochalasin-D 10 muM (ELF CB1 -/- +CYTOD); (n=3 different samples for each experimental group from 8 different animals in triplicate). qRT-PCR data are normalized using Cyclophilin and RPS18 , for SPZ and epididymis respectively, expressed as DeltaCq and reported as mean value +- S.E.M. Experimental groups with statistically significant differences (p<0.05; p<0.01) were indicated with different letters; the experimental groups without statistically significant differences were indicated with the same letter. White full arrowheads and asterisks represent F-actin localization (red) in sperm head and tail, respectively. Nuclei were labeled with DAPI (blue). Scale bar: 15 um (F) Immunofluorescence analysis of QKI protein in caput and cauda SPZ from WT and CB1 -/- mice. Whit

QKI Antibody in Western Blot (WB) — Characterization of backsplicing machinery in SPZ and epididymis of CTRL and HFD mice. ( A ) Immunofluorescence analysis of FUS protein in caput and cauda SPZ of CTRL and HFD mice. White arrowheads and white asterisks represent FUS localization (RED) in sperm head and tail, respectively. Nuclei were labeled with DAPI (blue). Scale bar: 5 um. ( B ) Western blot analysis of FUS protein (75 KDa) in caput and cauda SPZ of CTRL and HFD mice (n = 5 different samples for each experimental group in triplicate). Signals were quantified by densitometry analysis and normalized to Ponceau Red (Pon.S). Data were expressed in OD values and reported as mean +- SEM. Experimental groups with statistically significant differences ( p < 0.01) were indicated with different letters. ( C ) The enrichment levels of circADAM10 in the products of RIP assay (FUS-IP compared with IgG-IP) in caput and cauda SPZ of CTRL and HFD mice detected by qRT-PCR. Data are reported as mean +- SEM from three independent experiments. ** p < 0.01. ( D ) Immunocytochemistry of FUS in Bouin's fixed caput and cauda epididymis sections (7 mum) of CTRL and HFD mice (n = 5 different samples for each experimental group in triplicate). The FUS protein localization in principal cells was indicated by black arrows. Scale bar: 50 mum; inset: H&E staining of Bouin's fixed caput and cauda epididymis sections (7 mum) of CTRL and HFD mice; scale bar: 20 mum. ( E ) Western blot analysis of FUS protein in caput and cauda epididymis of

QKI Antibody in Western Blot, Immunoprecipitation (WB, IP) — Fig. 7 Human SPZ mimic mouse SPZ. qRT-PCR detection of circCNOT6L and CNOT6L-mRNA expression levels ( A ); immunoblots and quantification of CB1 ( B ), FUS ( C ) and ADAR ( D ) proteins in A- and B-SPZ fractions from normozoospermic volunteers ( n = 5 different samples in triplicate). Immunofluorescence analysis of FUS protein in A- and B-SPZ ( E and F ). White empty arrowheads and white full arrowheads represent FUS localization (FITC-green) in sperm head and tail, respectively. Nuclei were labeled with DAPI (blue). Scale bar: 20 uM. qRT-PCR detection of circCNOT6L and CNOT6L-mRNA expression levels ( G ) in B-SPZ fractions from normozoospermic volunteers ( n = 5) in vitro treated with vehicle (CTRL) or ACEA 1 uM ( n = 5 different samples for each experimental group in triplicate). In ( A ) and ( G ), the data are reported as mean value of nfe +- SEM, using Gapdh as endogenous control. In ( B ), ( C ) and ( D ) CB1, FUS and ADAR amount was quantified by densitometry analysis, normalized against Tubulin (TUB) signals, expressed as fc of OD values and reported as mean value +- SEM. ** P < 0.01. The enrichment levels of circCNOT6L and CNOT6L-mRNA in the products of RIP assay (FUS-IP compared with IgG-IP) in B-SPZ alone ( H ) and after in vitro ACEA treatment ( L ) by qRT-PCR. Data are reported as mean +- SEM from three independent experiments. ** P < 0.01. Western blot analysis of FUS, QKI and RNApol2 in the products of IP in B-SPZ ( I , J and K ) using FUS, QKI and RNApol2 anti

QKI Antibody in Western Blot, Immunoprecipitation (WB, IP) — Fig. 4 FUS drives CNOT6L backsplicing by interacting with RNApol2 and QKI in caput SPZ. The enrichment levels of circCNOT6L and CNOT6L-mRNA in the products of RIP assay (FUS-IP compared with IgG-IP) in WT caput SPZ alone ( A ) and after in vitro ACEA treatment ( F ) detected by qRT-PCR. Data are reported as mean +- SEM from three independent experiments. ** P < 0.01. qRT-PCR detection of circCNOT6L and CNOT6L-mRNA expression levels ( E ) in caput SPZ from WT mice in vitro treated with vehicle (CTRL) or ACEA 1 uM ( n = 3 different samples from three different animals for each experimental group in triplicate). Data are reported as mean value of nfe +- SEM, using Actin as endogenous control. ** P < 0.01. Western blot analysis of FUS, QKI and RNApol2 in the products of IP in WT caput SPZ ( B , C and D ) using FUS, QKI and RNApol2 antibodies. Western blot analysis of RIP protein fraction immunoprecipitated with FUS Ab (FUS-IP) in WT caput SPZ after in vitro ACEA treatment ( G ). FUS-IP is analyzed in comparison to control IgG-IP and Input protein extracts. qRT-PCR detection of Fus expression levels in ESCs, cultured in RM medium, treated with siScr or siFus (5, 10, 25 and 50 nM) and harvested after 48 ( H ) and 72 h ( I ) from siRNA transfection. Data are reported as mean value of nfe +- SEM, using Actin as endogenous control. ** P < 0.01; * P < 0.05. Western blot analysis of FUS protein ( J ) and qRT-PCR detection of circCNOT6L expression levels ( K ) in ESCs, cultured in RM med

产品信息

PA5-87292

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

查看3篇已发表文章 3篇已发表文章

免疫组化 (IHC)

-

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:50-1:200

查看2篇已发表文章 2篇已发表文章

免疫沉淀 (IP)

-

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant full length Human QKI.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 50% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2804039

产品详细信息

Purity is > 95% by SDS-PAGE.

靶标信息

This gene encodes the sacsin protein, which includes a UbL domain at the N-terminus, a DnaJ domain, and a HEPN domain at the C-terminus. The gene is highly expressed in the central nervous system, also found in skin, skeletal muscles and at low levels in the pancreas. This gene includes a very large exon spanning more than 12.8 kb. Mutations in this gene result in autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS), a neurodegenerative disorder characterized by early-onset cerebellar ataxia with spasticity and peripheral neuropathy. Sacsin has been found to protect against mutant ataxin-1. A pseudogene associated with this gene is located on chromosome 11. Alternative splicing of this gene results in multiple transcript variants.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Novel Insights into circRNA Saga Coming from Spermatozoa and Epididymis of HFD Mice.

International journal of molecular sciences

Manfrevola F,Chioccarelli T,Mele VG,Porreca V,Mattia M,Cimini D,D'Agostino A,Cobellis G,Fasano S,Schiraldi C,Chianese R,Pierantoni R

PA5-87292 was used in Western Blotting to demonstrate an enhanced skill of high-fat diet sperm in backsplicing activity together with an inefficient epididymal circRNA biogenesis.

Thu Apr 06 00:00:00 UTC 2023

Novel Insights into circRNA Saga Coming from Spermatozoa and Epididymis of HFD Mice.

International journal of molecular sciences

Manfrevola F,Chioccarelli T,Mele VG,Porreca V,Mattia M,Cimini D,D'Agostino A,Cobellis G,Fasano S,Schiraldi C,Chianese R,Pierantoni R

PA5-87292 was used in Western Blotting to demonstrate an enhanced skill of high-fat diet sperm in backsplicing activity together with an inefficient epididymal circRNA biogenesis.

Thu Apr 06 00:00:00 UTC 2023

Actin remodeling driven by circLIMA1: sperm cell as an intriguing cellular model.

International journal of biological sciences

Manfrevola F,Potenza N,Chioccarelli T,Di Palo A,Siniscalchi C,Porreca V,Scialla A,Mele VG,Petito G,Russo A,Lanni A,Senese R,Ricci G,Pierantoni R,Chianese R,Cobellis G

PA5-87292 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to show a sperm nuclear actin remodeling, consisting in the nuclear shuttling of circLIMA1-QKI interactome, and illustrate how endocannabinoids contribute to sperm morpho-cellular maturation.

Mon Aug 22 00:00:00 UTC 2022

Actin remodeling driven by circLIMA1: sperm cell as an intriguing cellular model.

International journal of biological sciences

Manfrevola F,Potenza N,Chioccarelli T,Di Palo A,Siniscalchi C,Porreca V,Scialla A,Mele VG,Petito G,Russo A,Lanni A,Senese R,Ricci G,Pierantoni R,Chianese R,Cobellis G

PA5-87292 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to show a sperm nuclear actin remodeling, consisting in the nuclear shuttling of circLIMA1-QKI interactome, and illustrate how endocannabinoids contribute to sperm morpho-cellular maturation.

Mon Aug 22 00:00:00 UTC 2022

Actin remodeling driven by circLIMA1: sperm cell as an intriguing cellular model.

International journal of biological sciences

Manfrevola F,Potenza N,Chioccarelli T,Di Palo A,Siniscalchi C,Porreca V,Scialla A,Mele VG,Petito G,Russo A,Lanni A,Senese R,Ricci G,Pierantoni R,Chianese R,Cobellis G

PA5-87292 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to show a sperm nuclear actin remodeling, consisting in the nuclear shuttling of circLIMA1-QKI interactome, and illustrate how endocannabinoids contribute to sperm morpho-cellular maturation.

Mon Aug 22 00:00:00 UTC 2022

Actin remodeling driven by circLIMA1: sperm cell as an intriguing cellular model.

International journal of biological sciences

Manfrevola F,Potenza N,Chioccarelli T,Di Palo A,Siniscalchi C,Porreca V,Scialla A,Mele VG,Petito G,Russo A,Lanni A,Senese R,Ricci G,Pierantoni R,Chianese R,Cobellis G

PA5-87292 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to show a sperm nuclear actin remodeling, consisting in the nuclear shuttling of circLIMA1-QKI interactome, and illustrate how endocannabinoids contribute to sperm morpho-cellular maturation.

Mon Aug 22 00:00:00 UTC 2022

Actin remodeling driven by circLIMA1: sperm cell as an intriguing cellular model.

International journal of biological sciences

Manfrevola F,Potenza N,Chioccarelli T,Di Palo A,Siniscalchi C,Porreca V,Scialla A,Mele VG,Petito G,Russo A,Lanni A,Senese R,Ricci G,Pierantoni R,Chianese R,Cobellis G

PA5-87292 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to show a sperm nuclear actin remodeling, consisting in the nuclear shuttling of circLIMA1-QKI interactome, and illustrate how endocannabinoids contribute to sperm morpho-cellular maturation.

Mon Aug 22 00:00:00 UTC 2022

Actin remodeling driven by circLIMA1: sperm cell as an intriguing cellular model.

International journal of biological sciences

Manfrevola F,Potenza N,Chioccarelli T,Di Palo A,Siniscalchi C,Porreca V,Scialla A,Mele VG,Petito G,Russo A,Lanni A,Senese R,Ricci G,Pierantoni R,Chianese R,Cobellis G

PA5-87292 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to show a sperm nuclear actin remodeling, consisting in the nuclear shuttling of circLIMA1-QKI interactome, and illustrate how endocannabinoids contribute to sperm morpho-cellular maturation.

Mon Aug 22 00:00:00 UTC 2022

FUS driven circCNOT6L biogenesis in mouse and human spermatozoa supports zygote development.

Cellular and molecular life sciences : CMLS

Chioccarelli T,Falco G,Cappetta D,De Angelis A,Roberto L,Addeo M,Ragusa M,Barbagallo D,Berrino L,Purrello M,Ambrosino C,Cobellis G,Pierantoni R,Chianese R,Manfrevola F

PA5-87292 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to suggest that spermatozoa (SPZ) have an endogenous skill to circularize mRNAs, choosing FUS as modulator of backsplicing and under CB1 stimulation.

Wed Dec 22 00:00:00 UTC 2021

FUS driven circCNOT6L biogenesis in mouse and human spermatozoa supports zygote development.

Cellular and molecular life sciences : CMLS

Chioccarelli T,Falco G,Cappetta D,De Angelis A,Roberto L,Addeo M,Ragusa M,Barbagallo D,Berrino L,Purrello M,Ambrosino C,Cobellis G,Pierantoni R,Chianese R,Manfrevola F

PA5-87292 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to suggest that spermatozoa (SPZ) have an endogenous skill to circularize mRNAs, choosing FUS as modulator of backsplicing and under CB1 stimulation.

Wed Dec 22 00:00:00 UTC 2021

FUS driven circCNOT6L biogenesis in mouse and human spermatozoa supports zygote development.

Cellular and molecular life sciences : CMLS

Chioccarelli T,Falco G,Cappetta D,De Angelis A,Roberto L,Addeo M,Ragusa M,Barbagallo D,Berrino L,Purrello M,Ambrosino C,Cobellis G,Pierantoni R,Chianese R,Manfrevola F

PA5-87292 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to suggest that spermatozoa (SPZ) have an endogenous skill to circularize mRNAs, choosing FUS as modulator of backsplicing and under CB1 stimulation.

Wed Dec 22 00:00:00 UTC 2021

生物信息学

蛋白别名: DKFZp586I0923; homolog of mouse quaking QKI (KH domain RNA binding protein); Hqk; KH domain-containing RNA-binding protein QKI; lethal, Chr 17, U Wisconsin 1; MqkI; Protein quaking; protein quaking-like; QKI, KH domain containing, RNA binding; QKI-5 protein; quaking homolog, KH domain RNA binding; RNA Binding (QKI); RNA binding protein HQK; rqkI

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基因别名: 1110003F05Rik; 1500005P18; HKQ; Hqk; hqkI; l(17)-1Wis; l17Wis1; QK; QK1; QK3; QKI

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UniProt ID: (Human) Q96PU8, (Mouse) Q9QYS9

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Entrez Gene ID: (Human) 9444, (Mouse) 19317, (Rat) 499022, (Rat) 100910212

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mRNA binding protein binding SH3 domain binding poly(A) RNA binding nucleic acid binding RNA binding RNA splicing factor

vasculogenesis mRNA processing spermatid development RNA splicing positive regulation of gene expression myelination muscle cell differentiation long-chain fatty acid biosynthetic process mRNA transport 3'-UTR-mediated mRNA destabilization regulation of translation transport multicellular organism development axon ensheathment cell differentiation