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QTRT1 Antibody (PA5-31270) in ICC/IF
QTRT1 Polyclonal Antibody detects QTRT1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: QTRT1 protein stained by QTRT1 Polyclonal Antibody (Product # PA5-31270) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-31270
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 22 and 340 of Human QTRT1
Conjugate
Form
Liquid
Concentration
0.89 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2548744
Product Specific Information
Recommended positive controls: NT2D1, PC-3.
Predicted reactivity: Mouse (89%), Rat (91%), Zebrafish (81%), Xenopus laevis (83%), Bovine (95%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
tRNA-guanine transglycosylase (TGT; EC 2.4.2.29) synthesizes queuosine (Q), which is found in tRNAs that recognize NAU and NAC codons, encoding tyr, asn, asp, and his. Prokaryotic TGT is a single protein of 43 kD. In contrast, mammalian TGT appears to be a heterodimer consisting of a 60-kD subunit (USP14; MIM 607274) and a 43-kD catalytic subunit (QTRT1) (Deshpande and Katze, 2001 [PubMed 11255023]).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Guanine insertion enzyme; queuine tRNA-ribosyltransferase; queuine tRNA-ribosyltransferase 1; Queuine tRNA-ribosyltransferase catalytic subunit 1; TGT, 43-KD subunit; TGT, catalytic subunit; tRNA-guanine transglycosylase
Gene Aliases: 2610028E17Rik; FP3235; QTRT1; TGT; TGUT
UniProt ID: (Human) Q9BXR0, (Mouse) Q9JMA2
Entrez Gene ID: (Human) 81890, (Mouse) 60507
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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