Invitrogen

Qa-2 Monoclonal Antibody (69H1-9-9), FITC, eBioscience™

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Qa-2 Antibody (11-5996-82) in Flow

Staining of C57BL/6 splenocytes with 0.25 µg of Mouse IgG2a kappa Isotype Control FITC (Product # 11-4724-42) (open histogram) or 0.25 µg of Anti-Mouse Qa-2 FITC (filled histogram). Total viable cells were used for analysis.

Product Image Gallery

Qa-2 Antibody — Figure 3 Thymic exosomes induce maturation of thymocytes. After three days in culture with thymic CD11c+ DCs and/or thymic exosomes (a), spleen exosomes (b), 100 nm latex beads (c), RNase treated thymic exosomes (d) or Proteinase K treated thymic exosomes (e); thymocytes were stained for surface expression of CD4+S1P1+Qa2+. After three days in culture with C57BL/6 or Balb/c thymic CD11c+ DCs, or C57BL/6 or Balb/c thymic exosomes, the C57BL/6 thymocytes were stained for surface expression of CD4+S1P1+Qa2+ (f). Left figure shows representative flow cytometry plots with indicated stimulations and surface markers. Right figure shows normalized flow cytometry data from at least three individual experiments. Data from exosome experiments are representative from at least three to five individual experiments. {TM}

产品信息

11-5996-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

查看8篇已发表文章 8篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

69H1-9-9

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_465352

产品详细信息

Description: The 69H1-9-9 monoclonal antibody reacts with the mouse Qa-2 molecule, a GPI-linked MHC class Ibeta surface molecule. Qa-2 is expressed by mature T and B cells, and its expression level is different depending on the laboratory mouse strain.

Applications Reported: The 69H1-9-9 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 69H1-9-9 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Qa-2 is a GPI-linked MHC class I beta surface molecule. Qa-2 is expressed by mature T and B cells, and its expression level varies between laboratory mouse strains.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Imprint of Initial Education and Loss of Ly49C/I in Activated Natural Killer Cells of TAP1-KO and C57BL/6 Wildtype Mice.

Frontiers in immunology

Patil ND,Domingues O,Masquelier C,Theresine M,Schlienger O,Njinju Amin Asaba C,Thomas M,Seguin-Devaux C,Slevogt H,Ollert M,Zimmer J

11-5996-82 was used in Flow cytometry/Cell sorting to show that we completed our investigations with phenotypic studies of NK cells from these mice.

Tue Aug 02 00:00:00 UTC 2022

Brief exposure to hyperglycemia activates dendritic cells in vitro and in vivo.

Journal of cellular physiology

Thomas AM,Dong Y,Beskid NM,García AJ,Adams AB,Babensee JE

11-5996-82 was used in Flow Cytometry to show that expression of the costimulatory molecule CD86 was found to reliably increase when dendritic cells were exposed to hyperglycemia.

Mon Jun 01 00:00:00 UTC 2020

Reduced expression of the murine HLA-G homolog Qa-2 is associated with malignancy, epithelial-mesenchymal transition and stemness in breast cancer cells.

Scientific reports

da Silva IL,Montero-Montero L,Martín-Villar E,Martin-Pérez J,Sainz B,Renart J,Toscano Simões R,Soares Veloso É,Salviano Teixeira C,de Oliveira MC,Ferreira E,Quintanilla M

11-5996 was used in Flow cytometry/Cell sorting to suggest an anti-malignant role for Qa-2 in breast cancer development.

Mon Jul 24 00:00:00 UTC 2017

NKT sublineage specification and survival requires the ubiquitin-modifying enzyme TNFAIP3/A20.

The Journal of experimental medicine

Drennan MB,Govindarajan S,Verheugen E,Coquet JM,Staal J,McGuire C,Taghon T,Leclercq G,Beyaert R,van Loo G,Lambrecht BN,Elewaut D

11-5996 was used in Flow cytometry/Cell sorting to show that negative regulation of TCR signalling during NKT development controls the differentiation and survival of NKT1 and NKT2 cells.

Mon Sep 19 00:00:00 UTC 2016

The thymic medulla is required for Foxp3+ regulatory but not conventional CD4+ thymocyte development.

The Journal of experimental medicine

Cowan JE,Parnell SM,Nakamura K,Caamano JH,Lane PJ,Jenkinson EJ,Jenkinson WE,Anderson G

11-5996 was used in Flow cytometry/Cell sorting to demonstrate a differential requirement for the thymic medulla in relation to CD4 conventional and Foxp3(+) thymocyte lineages.

Mon Apr 08 00:00:00 UTC 2013

Spontaneous cardiac calcinosis in BALB/cByJ mice.

Comparative medicine

Glass AM,Coombs W,Taffet SM

11-5996 was used in Flow cytometry/Cell sorting to suggest that immune cells are actively recruited to calcinotic lesions and that myocyte gap junctions are altered near calcinotic lesions.

Fri Feb 01 00:00:00 UTC 2013

Dynamic expression of Qa-2 during acute graft rejection.

Molecular medicine (Cambridge, Mass.)

Lu N,Wang C,Yang X,Zhao S,Li X,Li X,Jiang H,Feng J,Zhang Y,Zou X

11-5996 was used in Flow cytometry/Cell sorting to investigate the dynamic expression of Qa-2 in skin allografts.

Mon Aug 01 00:00:00 UTC 2011

The role of tapasin in MHC class I protein trafficking in embryos and T cells.

Journal of reproductive immunology

Lampton PW,Goldstein CY,Warner CM

11-5996 was used in Flow cytometry/Cell sorting to study the role of chaperone molecules in regulating Qa-2, an MHC class Ib protein, expression in early mice embryos.

Sun Jun 01 00:00:00 UTC 2008

生物信息学

蛋白别名: H2Q7; Qa-2 antigen

查看更多收起

基因别名: H2-Qa2; Ped; Qa-2

查看更多收起

Entrez Gene ID: (Mouse) 111458

查看更多收起

Qa-2 Monoclonal Antibody (69H1-9-9), FITC, eBioscience™

Qa-2 Antibody (11-5996-82) in Flow

产品信息

11-5996-82

流式细胞分析 (Flow)

种属反应

已发表种属

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

How to use the Panel Builder

生物信息学

性能保障

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