Invitrogen

RAB10 Monoclonal Antibody (4E2)

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查看其他7个RAB10抗体

抗体检测数据 (4)
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RAB10 Antibody in Immunocytochemistry (ICC/IF)

RAB10 Antibody in Immunocytochemistry (ICC/IF)

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RAB10 Antibody (MA5-15670) in ICC/IF

Immunofluorescence analysis of LOVO cells using Rab10 monoclonal antibody (Product # MA5-15670) (Green). Blue: DRAQ5 fluorescent DNA dye.

RAB10 Antibody in Immunocytochemistry (ICC/IF)

RAB10 Antibody in Western Blot (WB)

RAB10 Antibody in Western Blot (WB)

RAB10 Antibody in Western Blot (WB)

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RAB10 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of LOVO cells using Rab10 monoclonal antibody (Product # MA5-15670) (Green). Blue: DRAQ5 fluorescent DNA dye.

RAB10 Antibody in Western Blot (WB) — Western blot analysis was performed on membrane enriched cell extracts (30 µg lysate) of MCF-7 (Lane 1), A549 (Lane 2), HEK293 (Lane 3), HeLa (Lane 4), U-87 MG (Lane 5), U937 (Lane 6), A-431 (Lane 7), NIH3T3 (Lane 8) and tissue extract of Mouse Brain (Lane 9). The blot was probed with Anti-RAB10 Monoclonal Antibody (4E2) (Product # MA5-15670, 1:2000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 22 kDa band corresponding to RAB10 was observed across all the cell lines and tissue tested.

RAB10 Antibody in Western Blot (WB) — Western blot analysis of Rab10 using Rab10 monoclonal antibody (Product # MA5-15670) in HeLa (1) and NIH/3T3 (2) cell lysate.

RAB10 Antibody in Western Blot (WB) — Knockdown of RAB10 was achieved by transfecting HeLa cells with RAB10 specific siRNAs (Silencer® select Product # s21391, s21392). Western blot analysis (Fig. a) was performed using membrane enriched extracts from the RAB10 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with RAB10 Monoclonal Antibody (Product # MA5-15670, 1:2000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to RAB10.

RAB10 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with RAB10 siRNA and reduction of signal was observed in Western Blot using RAB10 Monoclonal Antibody (Product # MA5-15670). {KD}

产品信息

MA5-15670

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫细胞化学 (ICC/IF)

1:200-1:1,000

查看1篇已发表文章 1篇已发表文章

酶联免疫吸附实验 (ELISA)

1:10,000

-

产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

4E2

抗原

Purified recombinant fragment of human Rab10 expressed in E. Coli.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

ascites

内含物

0.03% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_10981668

产品详细信息

MA5-15670 targets Rab10 in indirect ELISA, IF and WB applications and shows reactivity with Human and mouse samples.

The MA5-15670 immunogen is purified recombinant fragment of human Rab10 expressed in E. Coli.

.

MA5-15670 detects Rab10 which has a predicted molecular weight of approximately 22.5kDa.

靶标信息

RAB10 belongs to the RAS superfamily of small GTPases. RAB proteins localize to exocytic and endocytic compartments and regulate intracellular vesicle trafficking.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

RAB8, RAB10 and RILPL1 contribute to both LRRK2 kinase-mediated centrosomal cohesion and ciliogenesis deficits.

Human molecular genetics

Lara Ordónez AJ,Fernández B,Fdez E,Romo-Lozano M,Madero-Pérez J,Lobbestael E,Baekelandt V,Aiastui A,López de Munaín A,Melrose HL,Civiero L,Hilfiker S

MA5-15670 was used in Immunocytochemistry to show that both RAB8 and RAB10 contribute to the centrosomal cohesion deficits.

Fri Nov 01 00:00:00 UTC 2019

生物信息学

蛋白别名: GTP-binding protein RAB10; ras-related GTP-binding protein; Ras-related protein Rab-10

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基因别名: AW107754; RAB10

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UniProt ID: (Human) P61026, (Mouse) P61027

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Entrez Gene ID: (Human) 10890, (Mouse) 19325

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GTPase activity protein binding GTP binding GDP binding myosin V binding cadherin binding involved in cell-cell adhesion nucleotide binding

transport intracellular protein transport Golgi to plasma membrane transport vesicle docking involved in exocytosis small GTPase mediated signal transduction axonogenesis metabolic process protein secretion protein transport vesicle-mediated transport endosomal transport regulation of exocytosis antigen processing and presentation Rab protein signal transduction cellular response to insulin stimulus establishment of neuroblast polarity endoplasmic reticulum tubular network organization protein localization to plasma membrane establishment of protein localization to membrane establishment of protein localization to endoplasmic reticulum membrane polarized epithelial cell differentiation Golgi to plasma membrane protein transport membrane organization basolateral protein localization cellular response to antibiotic cell-cell adhesion