Invitrogen

RAB12 Polyclonal Antibody

RAB12 Antibody in Immunocytochemistry (ICC/IF)

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RAB12 Antibody (PA5-48179) in ICC/IF

Immunofluorescence analysis of RAB12 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with RAB12 Polyclonal Antibody (Product # PA5-48179, 1:100) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:200... View More

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RAB12 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with RAB12 siRNA and loss of signal was observed in Western Blot application using RAB12 Polyclonal Antibody (Product # PA5-48179). {KD}

产品信息

PA5-48179

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2000

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

1:25

产品规格

种属反应

Human, Mouse

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 63-92 amino acids from the N-terminal region of human RAB12.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2633637

产品详细信息

Predicted to react with rat based on sequence homology.

靶标信息

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab may play a role in protein transport from recycling endosomes to lysosomes regulating, for instance, the degradation of the transferrin receptor. Involved in autophagy.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

SLC13A5/sodium-citrate co-transporter overexpression causes disrupted white matter integrity and an autistic-like phenotype.

Brain communications

Rigby MJ,Orefice NS,Lawton AJ,Ma M,Shapiro SL,Yi SY,Dieterich IA,Frelka A,Miles HN,Pearce RA,Yu JPJ,Li L,Denu JM,Puglielli L

PA5-48179 was used in Western Blot to test the hypothesis that modulating the intracellular flux of citrate through overexpression of SLC13A5, the plasma membrane sodium/citrate symporter, would result in an autistic-like phenotype in a mouse model.

Tue Feb 01 00:00:00 UTC 2022

Increased expression of SLC25A1/CIC causes an autistic-like phenotype with altered neuron morphology.

Brain : a journal of neurology

Rigby MJ,Orefice NS,Lawton AJ,Ma M,Shapiro SL,Yi SY,Dieterich IA,Frelka A,Miles HN,Pearce RA,Yu JPJ,Li L,Denu JM,Puglielli L

PA5-48179 was used in Western Blotting to reinforce the connection between aberrant cytosolic-to-endoplasmic reticulum acetyl-CoA flux and the development of an autistic-like phenotype.