Invitrogen

RAB17 Polyclonal Antibody

查看其他14个RAB17抗体

抗体检测数据 (5)

RAB17 Antibody in Immunocytochemistry (ICC/IF)

RAB17 Antibody in Immunocytochemistry (ICC/IF)

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RAB17 Antibody (PA5-115816) in ICC/IF

Immunocytochemistry analysis of RAB17 in A549 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115816). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.

RAB17 Antibody in Immunocytochemistry (ICC/IF)

RAB17 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAB17 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAB17 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAB17 Antibody in Western Blot (WB)

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RAB17 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of RAB17 in A549 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115816). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.

RAB17 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RAB17 in human ovarian cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with RAB17 polyclonal antibody (Product # PA5-115816) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

RAB17 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RAB17 in rat testis tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with RAB17 polyclonal antibody (Product # PA5-115816) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

RAB17 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RAB17 in rat brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with RAB17 polyclonal antibody (Product # PA5-115816) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

RAB17 Antibody in Western Blot (WB) — Western blot analysis of RAB17 in HUVEC whole cells lysates. Samples were incubated with polyclonal antibody (Product # PA5-115816).

产品信息

PA5-115816

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human RAB17(Accession Q9H0T7), corresponding to amino acid residues S82-N132.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2900450

产品详细信息

Antibody detects endogenous levels of total RAB17.

靶标信息

The Rab subfamily of small GTPases plays an important role in the regulation of membrane trafficking. RAB17 is an epithelial cell-specific GTPase.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Ras-related protein Rab-17

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基因别名: AW413472; RAB17; RGD1562478

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UniProt ID: (Human) Q9H0T7, (Mouse) P35292

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Entrez Gene ID: (Human) 64284, (Mouse) 19329, (Rat) 503269

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GTPase activity protein binding GTP binding GDP binding nucleotide binding

immunoglobulin transcytosis in epithelial cells mediated by polymeric immunoglobulin receptor transport intracellular protein transport endocytosis small GTPase mediated signal transduction metabolic process protein transport regulation of endocytosis establishment of melanosome localization melanosome transport endocytic recycling Rab protein signal transduction cilium assembly transcytosis filopodium assembly regulation of dendrite development regulation of filopodium assembly regulation of synapse assembly