Invitrogen

RAB21 Monoclonal Antibody (OTI6E12)

RAB21 Antibody in Immunocytochemistry (ICC/IF)

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RAB21 Antibody (MA5-26200) in ICC/IF

Immunofluorescence analysis of RAB21 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with RAB21 Monoclonal Antibody (OTI6E12) (Product # MA5-26200) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32766)... View More

RAB21 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

MA5-26200

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:200-1:2,000

免疫组化（石蜡） (IHC (P))

1:150

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Dog, Human, Mouse, Non-human primate, Rat

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

OTI6E12

抗原

Full length human recombinant protein of RAB21 produced in HEK293T cell

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 1% BSA, 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2723544

靶标信息

RAB21 is a member of a subfamily of small GTP-binding protein genes of the Ras superfamily that plays an important role in intracellular vesicular targeting. In non-polarized Caco-2 cells, RAB21 showed a general endoplasmic reticulum (ER)-like localization, while in polarized cells, RAB21 localized to apical vesicles. RAB21 has been shown to associate with integrin subunits and to be important for receptor entry into cells via RAB5/RAB21 endosomes. The GTPase-activating protein p120RasGAP regulates cell motility by controlling the return of the endocytosed integrins to the plasma membrane by competing with RAB21 for binding to overlapping sites on the Alpha-tail of endocytosed integrin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。