Invitrogen

RAB34 Polyclonal Antibody

RAB34 Antibody in Immunocytochemistry (ICC/IF)

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RAB34 Antibody (PA5-99697) in ICC/IF

Immunocytochemistry analysis of RAB34 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with RAB34 polyclonal antibody (Product # PA5-99697) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

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产品信息

PA5-99697

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human RAB34(Accession Q9BZG1), corresponding to amino acid residues K144-Y194.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2818630

产品详细信息

Antibody detects endogenous levels of total Rab 34.

靶标信息

Protein transport. Involved in the redistribution of lysosomes to the peri-Golgi region. Plays a role in the maturation of phagosomes that engulf pathogens, such as S. aureus and M. tuberculosis. Plays a role in the fusion of phagosomes with lysosomes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

E2F1‑mediated RAB34 upregulation accelerates the proliferation and inhibits the cell cycle arrest and apoptosis of acute myeloid leukemia cells.

Experimental and therapeutic medicine

Xiong X,Jian G

PA5-99697 was used in Western Blotting to suggest that E2F1-mediated RAB34 upregulation may accelerate the malignant progression of AML.

Tue Aug 01 00:00:00 UTC 2023

E2F1‑mediated RAB34 upregulation accelerates the proliferation and inhibits the cell cycle arrest and apoptosis of acute myeloid leukemia cells.

Experimental and therapeutic medicine

Xiong X,Jian G

PA5-99697 was used in Western Blotting to suggest that E2F1-mediated RAB34 upregulation may accelerate the malignant progression of AML.

Tue Aug 01 00:00:00 UTC 2023

生物信息学

蛋白别名: Nine amino-acid residue-repeats; RAB34, member of RAS oncogene family; Ras-related protein Rab-34; Ras-related protein Rab-34, isoform NARR; Ras-related protein Rab-39; Ras-related protein RAB34; Ras-related protein Rah

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基因别名: NARR; RAB34; RAB39; RAH

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UniProt ID: (Human) P0DI83, (Rat) Q5U1Y1

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Entrez Gene ID: (Human) 83871, (Rat) 360571

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功能

protein binding GTP binding Ral GTPase binding GTPase activity guanyl nucleotide binding GDP binding

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