Invitrogen

RAB35 Polyclonal Antibody

查看其他4个RAB35抗体

抗体检测数据 (5)

RAB35 Antibody in Immunocytochemistry (ICC/IF)

RAB35 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 5

RAB35 Antibody (PA5-103244) in ICC/IF

Immunofluorescent analysis of RAB35 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with RAB35 polyclonal antibody (Product # PA5-103244) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

RAB35 Antibody in Immunocytochemistry (ICC/IF)

RAB35 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAB35 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAB35 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAB35 Antibody in Western Blot (WB)

Product Image Gallery

RAB35 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of RAB35 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with RAB35 polyclonal antibody (Product # PA5-103244) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

RAB35 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RAB35 in mouse testis tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with RAB35 polyclonal antibody (Product # PA5-103244) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

RAB35 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RAB35 in mouse kidney tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with RAB35 polyclonal antibody (Product # PA5-103244) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

RAB35 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RAB35 in rat kidney tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with RAB35 polyclonal antibody (Product # PA5-103244) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

RAB35 Antibody in Western Blot (WB) — Western blot analysis of RAB35 in HepG2 cell lysate (Lane 1: treated with blocking peptide). Samples were incubated with RAB35 polyclonal antibody (Product # PA5-103244).

产品信息

PA5-103244

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human RAB35(Accession Q15286), corresponding to amino acid residues N98-E148.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2852608

产品详细信息

Antibody detects endogenous levels of total RAB35.

靶标信息

RAB35 possesses GTPase activity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: GTP-binding protein RAY; Ras-related protein Rab-1C; ras-related protein rab-1c (GTP-binding protein ray); Ras-related protein Rab-35

查看更多收起

基因别名: 9530019H02Rik; AU040256; H-ray; RAB1C; RAB35; RAY

查看更多收起

UniProt ID: (Human) Q15286, (Mouse) Q6PHN9, (Rat) Q5U316

查看更多收起

Entrez Gene ID: (Human) 11021, (Mouse) 77407, (Rat) 288700

查看更多收起

GTPase activity protein binding GTP binding phosphatidylinositol-4,5-bisphosphate binding GDP binding nucleotide binding

cytokinesis ER to Golgi vesicle-mediated transport small GTPase mediated signal transduction protein localization protein transport endosomal transport antigen processing and presentation neuron projection development endocytic recycling protein localization to endosome plasma membrane to endosome transport cellular response to nerve growth factor stimulus transport intracellular protein transport metabolic process Rab protein signal transduction