Invitrogen

RAB3A Recombinant Rabbit Monoclonal Antibody (PSH01-08)

RAB3A Antibody in Immunocytochemistry (ICC/IF)

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RAB3A Antibody (MA5-49607) in ICC/IF

Immunofluorescence of RAB3A was performed using SK-N-FI WT and RAB3A KO cells that were labeled with a green or a far-red fluorescent dye, respectively. Post-labeling, WT and KO cells were mixed and plated to a 1:1 ratio in a 96-well plate with an optically clear flat bottom as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) for 15 min at RT; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS containing 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min at RT. Cells were stain... View More

RAB3A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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RAB3A Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues owing to their inherent genetic constitution. Relative expression of RAB3A was observed in Mouse Brain and Rat Brain in comparison to Mouse Kidney, Mouse Liver, Rat Kidney and Rat Liver using RAB3A Rabbit Recombinant Monoclonal Antibody (PSH01-080) (Product # MA5-49607) in western blot (10.1128/MCB.22.6.1858-1867.2002). {RE}

产品信息

MA5-49607

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000

免疫组化（石蜡） (IHC (P))

1:1,000

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

1:500-1:1,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

PSH01-08

抗原

Synthetic peptide within human rab3a aa 121-170/220

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_3094127

产品详细信息

Sequence Similarities: 100% Mouse/Rat.

Tissue Specificity: Specifically expressed in brain.

Positive Control: Human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, human cerebellum tissue, mouse cerebellum tissue, rat cerebellum tissue, SH-SY5Y, N2A, PC-12.

Subcellular Location: Cytoplasm, cytosol, Lysosome, Cytoplasmic vesicle, secretory vesicle, Cell projection, axon, Cell membrane, Presynapse, Postsynapse.

Predicted band size: 25 kDa

靶标信息

Rab proteins belong to the Ras small GTPase superfamily. The GTP-binding Rab3 subfamily has 4 known isoforms: 3A, 3B, 3C, and 3D. These proteins are expressed in highest concentrations in brain and endocrine tissues and involved in regulated exocytosis of hormones and neurotransmitters. Specifically, Rab 3s are thought to play a key role in presynaptic vesicle trafficking and priming.

仅用于科研。不用于诊断过程。未经明确授权不得转售。