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RAB3d Antibody (PA1-773) in ICC/IF
Immunofluorescence analysis of RAB3D was performed using 70% confluent log phase 3T3-L1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with RAB3D Rabbit Polyclonal Antibody (Product # PA1-773) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A... View More
产品信息
PA1-773
产品规格
种属反应
Mouse,
Rat
已发表种属
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues M(1) A S A S E P P A S P R D A A D Q N(18) of rat RAB3D.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2177236
产品详细信息
PA1-773 detects recombinant rat and mouse rab 3D. This antibody does not detect rab 3A, 3B, or 3C.
PA1-773 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~29 kDa protein representing recombinant mouse rab 3D.
PA1-773 immunogen is a synthetic peptide corresponding to residues M(1) A S A S E P P A S P R D A A D Q N(18) of rat RAB3D. This sequence is completely conserved in mouse. This immunizing peptide (Cat. # PEP-144) is available for use in neutralization and control experiments.
靶标信息
Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 rab proteins that have been described in mammals. Rab 3D has been shown to be present, in part, in the tongue, pancreas, and liver.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: GTP-binding protein Rab-3D; Ras-related protein Rab-3D
基因别名: C130057E11Rik; Rab16; Rab3d
UniProt ID: (Mouse) P35276, (Rat) Q63942
Entrez Gene ID: (Mouse) 19340, (Rat) 140665
transport
intracellular protein transport
exocytosis
vesicle docking involved in exocytosis
small GTPase mediated signal transduction
protein secretion
protein transport
synaptic vesicle exocytosis
regulation of exocytosis
peptidyl-cysteine methylation
Rab protein signal transduction
positive regulation of regulated secretory pathway
protein localization to plasma membrane
