Invitrogen

RAB5A Monoclonal Antibody (2E8B11), eBioscience™

RAB5A Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 6

RAB5A Antibody (14-9711-82) in ICC/IF

For immunofluorescence analysis, HeLa cells were fixed for detection of endogenous Rab5a using Anti-Rab5a Monoclonal Antibody (Product # 14-9711-82, 1:100 dilution) and labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766, 1:2000). Panel a) shows representative cells that were stained for detection of Rab5a protein (green), Panel b) is stained for nuclei (blue) using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). Panel c) represents cytoskeletal F-actin ... View More

RAB5A Antibody in Immunoprecipitation (IP)

Product Image Gallery

RAB5A Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Rab5a siRNA (Silencer® select Product # s11679; s11680) and decrease in signal intensity was observed in western blot application using Anti-Rab5a Monoclonal Antibody. Western blot analysis (Fig. a) was performed using whole cell extracts from cells transfected with Rab5a siRNA (lane 3), non-specific scrambled siRNA (lane 2) or left untransfected (lane 1). The blot was probed with Anti-Rab5a Monoclonal Antibody (Product # 14-9711-82, 1:1000 dilution) and Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in the histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that the antibody is specific to Rab5a. {KD}

产品信息

14-9711-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫细胞化学 (ICC/IF)

1:100

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

2E8B11

抗原

Purified recombinant fragment of human Rab5a (AA: 1-215) expressed in E. Coli.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_2884043

产品详细信息

Description: This 2E8B11 monoclonal antibody recognizes human Rab5a, which is a GTPase involved in intracellular membrane trafficking. Rab5a is reported to have ubiquitous expression.

Applications Reported: The 2E8B11 clone has been reported for use in intracellular flow cytometry, western blot, ELISA, immunocytochemistry of cells and immunohistochemistry of tissue.

Applications Tested: This 2E8B11 antibody has been tested by western blot in multiple human cell lines.
Applications Tested: This 2E8B11 antibody has been tested by immunocytochemical staining of fixed and permeabilized HeLa cells.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

RAB5 are among the small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan. Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3.

仅用于科研。不用于诊断过程。未经明确授权不得转售。