Invitrogen

RAB8A Monoclonal Antibody (261CT1.3.1)

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RAB8A Antibody (MA5-24717) in WB

Western blot was performed using RAB8A Monoclonal Antibody (261CT1.3.1) (Product # MA5-24717) and a 22 kDa band corresponding to RAB8A was observed. For Fig a, whole cell extracts (30 µg lysate) of MCF7 (Lane 1), BT-549 (Lane 2), HeLa (Lane 3), HCT 116 (Lane 4), Raji (Lane 5), HEL 92.1.7 (Lane 6), A549 (Lane 7) and for Fig b, tissue extracts (30 µg lysate) of Rat Ileum (Lane 1) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX), 12 well. Resolved proteins were then transferred onto a nitrocellulose membrane (... View More

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RAB8A Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with RAB8A siRNA and decrease in signal intensity was observed in Western Blot application using RAB8A Monoclonal Antibody (261CT1.3.1) (Product # MA5-24717). {KD}

产品信息

MA5-24717

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:1,000

产品规格

种属反应

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

261CT1.3.1

抗原

human RAB8A recombinant protein

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2717192

靶标信息

The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: cell line NK14 derived transforming oncogene; mel transforming oncogene (derived from cell line NK14); mel transforming oncogene (derived from cell line NK14)- RAB8 homolog; mel transforming oncogene (RAB8 homolog); Oncogene c-mel; ras-associated protein RAB8; Ras-related protein Rab-8A

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基因别名: AA409338; MEL; RAB8; RAB8A

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UniProt ID: (Human) P61006, (Mouse) P55258

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Entrez Gene ID: (Human) 4218, (Mouse) 17274

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功能

GTPase activity protein binding GTP binding Rab GTPase binding GDP binding protein kinase binding myosin V binding nucleotide binding

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