Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 8
RAB8A Antibody (MA5-49140) in ICC/IF
Immunofluorescence analysis of RAB8A was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with RAB8A Rabbit Recombinant Monoclonal Antibody (JE64-79) (Product # MA5-49140 ) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluo... View More
产品信息
MA5-49140
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JE64-79
抗原
Synthetic peptide within human rab8a aa 151-200/207.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_3074798
产品详细信息
Sequence Similarities: 100% Mouse/Rat
Tissue Specificity: Low tissue specificity
Positive Control: Hela cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C2C12 cell lysate, L6 cell lysate
Subcellular location: Cell membrane, golgi apparatus, recycling endosome membrane, cytoplasm, centriole, cilium basal body, cilium axoneme, cilium, phagosome, phagosome membrane, midbody.
靶标信息
The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: cell line NK14 derived transforming oncogene; mel transforming oncogene (derived from cell line NK14); mel transforming oncogene (derived from cell line NK14)- RAB8 homolog; mel transforming oncogene (RAB8 homolog); Oncogene c-mel; ras-associated protein RAB8; Ras-related protein Rab-8A
基因别名: AA409338; MEL; RAB8; RAB8A
UniProt ID: (Human) P61006, (Mouse) P55258, (Rat) P35280
Entrez Gene ID: (Human) 4218, (Mouse) 17274, (Rat) 117103
G2/M transition of mitotic cell cycle
vesicle docking involved in exocytosis
small GTPase mediated signal transduction
axonogenesis
protein secretion
cellular response to insulin stimulus
cilium assembly
regulation of long-term neuronal synaptic plasticity
Golgi vesicle fusion to target membrane
regulation of protein transport
membrane organization
protein localization to plasma membrane
transport
intracellular protein transport
metabolic process
protein transport
synaptic vesicle exocytosis
regulation of exocytosis
cell projection organization
Rab protein signal transduction
regulation of protein localization
glucose homeostasis
positive regulation of glucose import
