Invitrogen

RAB9B Polyclonal Antibody

查看其他7个RAB9B抗体

抗体检测数据 (6)

RAB9B Antibody in Immunocytochemistry (ICC/IF)

RAB9B Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 6

RAB9B Antibody (PA5-104086) in ICC/IF

Immunocytochemistry analysis of RAB9B in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with RAB9B polyclonal antibody (Product # PA5-104086) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

RAB9B Antibody in Immunocytochemistry (ICC/IF)

RAB9B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAB9B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAB9B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAB9B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAB9B Antibody in Western Blot (WB)

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RAB9B Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of RAB9B in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with RAB9B polyclonal antibody (Product # PA5-104086) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

RAB9B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RAB9B in mouse muscle tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with RAB9B polyclonal antibody (Product # PA5-104086) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

RAB9B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RAB9B in rat spleen tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with RAB9B polyclonal antibody (Product # PA5-104086) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

RAB9B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RAB9B in rat muscle tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with RAB9B polyclonal antibody (Product # PA5-104086) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

RAB9B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RAB9B in rat skin tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with RAB9B polyclonal antibody (Product # PA5-104086) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

RAB9B Antibody in Western Blot (WB) — Western blot analysis of Rab9 in rat brain (Lane 1: treated with blocking peptide). Samples were incubated with Rab9 polyclonal antibody (Product # PA5-104086).

产品信息

PA5-104086

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human RAB9B(Accession Q9NP90), corresponding to amino acid residues E104-S154.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2853414

产品详细信息

Antibody detects endogenous levels of total RAB9B.

靶标信息

Rab9b may be involved in the transport of proteins between the endosomes and the trans Golgi network.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Rab-9-like protein; Rab-9L; Ras-related protein Rab-9B; TRAB9

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基因别名: 9330195C02Rik; Rab-9L; RAB9B; RAB9L; RGD1562951

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UniProt ID: (Human) Q9NP90, (Mouse) Q8BHH2

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Entrez Gene ID: (Human) 51209, (Rat) 367915, (Mouse) 319642

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protein binding GTP binding GDP binding GTPase activity nucleotide binding

intracellular protein transport metabolic process Rab protein signal transduction retrograde transport, endosome to Golgi phagosome-lysosome fusion small GTPase mediated signal transduction protein transport transport