Invitrogen

RACK1 Polyclonal Antibody

RACK1 Antibody in Immunocytochemistry (ICC/IF)

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RACK1 Antibody (PA5-17484) in ICC/IF

Immunofluorescence analysis of RACK1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with RACK1 Polyclonal Antibody (Product # PA5-17484) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2703... View More

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RACK1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with RACK1 siRNA and reduction of signal was observed in Western Blot using RACK1 Polyclonal Antibody (Product # PA5-17484). {KD}

产品信息

PA5-17484

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫细胞化学 (ICC/IF)

1:200

产品规格

种属反应

Fruit fly, Human, Mouse, Rat, Zebrafish

已发表种属

Yeast

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to human RACK1

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

29 µg/mL

纯化类型

Antigen affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10980517

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

RACK1 (receptor for activated C kinase 1) was identified through its binding to various PKC isoforms. Its main function is to recruit PKC and various other proteins to specific locations to form multiprotein complexes, mediating various signal pathways. RACK1 is required for VANGL2 membrane localization and for PTK2/FAK1 phosphorylation and dephosphorylation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Communication between RACK1/Asc1 and uS3 (Rps3) is essential for RACK1/Asc1 function in yeast Saccharomyces cerevisiae.

Gene

Singh N,Jindal S,Ghosh A,Komar AA

PA5-17484 was used in Western Blotting to suggest that eukaryote-specific RACK1-uS3 interaction has evolved to act as a link between the ribosome and the cellular signaling pathways.

Sat Jul 20 00:00:00 UTC 2019

生物信息学

蛋白别名: 12-3; activated protein kinase C receptor; Cell proliferation-inducing gene 21 protein; CG7111-PA; CG7111-PB; CG7111-PC; CG7111-PD; guanine nucleotide binding protein (G protein) beta polypeptide 2-like 1; guanine nucleotide binding protein (G protein), beta polypeptide 2 like 1 sequence 1; guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1; guanine nucleotide binding protein beta polypeptide 2-like 1; guanine nucleotide binding protein related; guanine nucleotide binding protein, beta 2, related sequence 1; guanine nucleotide binding protein, beta polypeptide 2-like 1; guanine nucleotide binding protein, beta-2, related sequence 1; guanine nucleotide-binding protein beta-subunit like protein; Guanine nucleotide-binding protein subunit beta-2-like 1; Guanine nucleotide-binding protein subunit beta-like protein; Guanine nucleotide-binding protein subunit beta-like protein 12.3; HLC-7; Human lung cancer oncogene 7 protein; lung cancer oncogene 7; OTTHUMP00000223870; OTTHUMP00000223891; OTTHUMP00000223893; OTTHUMP00000223900; OTTHUMP00000223901; OTTHUMP00000223902; OTTHUMP00000223930; OTTHUMP00000223931; p205; proliferation-inducing gene 21; protein homologous to chicken B complex protein, guanine nucleotide binding; protein kinase C receptor; RACK; rack-1; Rack1-PA; Rack1-PB; Rack1-PC; Rack1-PD; RE74715p; Receptor for activated C kinase; receptor for activated C kinase 1; receptor for activated protein kinase C; receptor for activated protein kinase C1; receptor of activated PKC; Receptor of activated protein C kinase 1; Receptor of activated protein kinase C; Receptor of activated protein kinase C 1; Receptor of activated protein kinase C homolog; similar to mammalian RACK1; Small ribosomal subunit protein RACK1

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基因别名: AL033335; CG7111; Dmel\CG7111; Dmel_CG7111; dRack1; GB-like; Gnb2-rs1; GNB2L1; H12.3; HLC-7; HLC7; i173; p205; PIG21; RACK1; wu:fb80d08; wu:fk65d12