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RAD1 Antibody (702149) in ICC/IF
For immunofluorescence analysis, HeLa cells were fixed and permeabilized for detection of endogenous Rad1 using Rad1 Recombinant Rabbit Monoclonal Antibody (Product # 702149, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Rad1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel... View More
产品信息
702149
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
8H19L13
抗原
Protein corresponding to Human RAD1 (aa 133-282)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2633061
产品详细信息
This antibody is predicted to react with Monkey, Pig, Cat and Sheep.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
Rad1 is a component of a heterotrimeric PCNA-like complex that contains Rad9 and Hus1 proteins. This complex is involved in the 9-1-1 cell cycle checkpoing response complex that plays a major role in DNA repair, by associating with Rad17 and several components of the PCNA-loading heteropentamer replication factor C. Mutants has been shown to partially restore radiation resistance and G2-checkpoint activity. It has also been shown to possess exonuclease activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: cell cycle checkpoint protein Hrad1; Cell cycle checkpoint protein RAD1; checkpoint control protein HRAD1; DNA repair exonuclease rad1 homolog; DNA repair exonuclease REC1; exonuclease homolog RAD1; hRAD1; RAD1 checkpoint clamp component; RAD1 homolog; Rad1-like DNA damage checkpoint protein
基因别名: HRAD1; RAD1; REC1
UniProt ID: (Human) O60671
Entrez Gene ID: (Human) 5810
