Invitrogen

RAD50 Polyclonal Antibody

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RAD50 Antibody (PA5-32176) in WB

Western Blot using RAD50 Polyclonal Antibody (Product # PA5-32176). Sample (10 µg of whole cell lysate). Lane A: Yeast . 5% SDS PAGE. RAD50 Polyclonal Antibody (Product # PA5-32176) diluted at 1:1,000.

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产品信息

PA5-32176

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看4篇已发表文章 4篇已发表文章

产品规格

种属反应

Yeast

已发表种属

Insect, Yeast

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 644 and 989 of Yeast RAD50

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2549649

产品详细信息

Recommended positive controls: Yeast.

Predicted reactivity: Yeast (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Rad50-13B3 recognizes human Rad50, a 153a protein involved in DNA double strand break repair. Rad50 is associated with Mre11 and p95 (Nibrin) to form a multiprotein complex involved in the double strand break repair process. Upon DNA damage, Rad50 and Mre11 redistribute and are co-localized within the nucleus of damaged cells.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Mre11-Rad50 oligomerization promotes DNA double-strand break repair.

Nature communications

Kissling VM,Reginato G,Bianco E,Kasaciunaite K,Tilma J,Cereghetti G,Schindler N,Lee SS,Guérois R,Luke B,Seidel R,Cejka P,Peter M

PA5-32176 was used in Western Blot to demonstrate that Mre11-Rad50-Xrs2 oligomerization facilitates foci formation, DNA damage signaling, repair, and telomere maintenance in vivo.

Mon May 02 00:00:00 UTC 2022

Inhibition of MRN activity by a telomere protein motif.

Nature communications

Khayat F,Cannavo E,Alshmery M,Foster WR,Chahwan C,Maddalena M,Smith C,Oliver AW,Watson AT,Carr AM,Cejka P,Bianchi A

PA5-32176 was used in Western Blot to raise the possibility that analogous mechanisms might be deployed in other eukaryotes as well.

Tue Jun 22 00:00:00 UTC 2021

Inhibition of MRN activity by a telomere protein motif.

Nature communications

Khayat F,Cannavo E,Alshmery M,Foster WR,Chahwan C,Maddalena M,Smith C,Oliver AW,Watson AT,Carr AM,Cejka P,Bianchi A

PA5-32176 was used in Western Blot to raise the possibility that analogous mechanisms might be deployed in other eukaryotes as well.

Tue Jun 22 00:00:00 UTC 2021

Sae2 and Rif2 regulate MRX endonuclease activity at DNA double-strand breaks in opposite manners.

Cell reports

Marsella A,Gobbini E,Cassani C,Tisi R,Cannavo E,Reginato G,Cejka P,Longhese MP

PA5-32176 was used in Western Blot to show that the Rif2 protein inhibits Mre11 endonuclease activity and is responsible for the increased MRX retention at DSBs in sae2Δ cells.

Tue Mar 30 00:00:00 UTC 2021

Sae2 and Rif2 regulate MRX endonuclease activity at DNA double-strand breaks in opposite manners.

Cell reports

Marsella A,Gobbini E,Cassani C,Tisi R,Cannavo E,Reginato G,Cejka P,Longhese MP

PA5-32176 was used in Western Blot to show that the Rif2 protein inhibits Mre11 endonuclease activity and is responsible for the increased MRX retention at DSBs in sae2Δ cells.

Tue Mar 30 00:00:00 UTC 2021

Regulatory control of DNA end resection by Sae2 phosphorylation.

Nature communications

Cannavo E,Johnson D,Andres SN,Kissling VM,Reinert JK,Garcia V,Erie DA,Hess D,Thomä NH,Enchev RI,Peter M,Williams RS,Neale MJ,Cejka P

PA5-32176 was used in Western Blotting to define how phosphorylation controls the initiation of DNA end resection and therefore the choice between the key DNA double-strand break repair mechanisms.