Invitrogen

RAD51 Polyclonal Antibody

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RAD51 Antibody (PA5-34905) in WB

Western Blot using RAD51 Polyclonal Antibody (Product # PA5-34905). Sample (10 µg of whole cell lysate). Lane A: yeast . 7.5% SDS PAGE. RAD51 Polyclonal Antibody (Product # PA5-34905) diluted at 1:1,000.

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产品信息

PA5-34905

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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染色质免疫沉淀实验 (ChIP)

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产品规格

种属反应

Yeast

已发表种属

Yeast

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 1 and 259 of RAD51 (Uniprot ID#P25454)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2552256

产品详细信息

Recommended positive controls: yeast.

Predicted reactivity: Yeast (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recobination. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to a single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Mutations in the gene can results in breast cancer, mirror movements 2 and fanconi anemia.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Structure-forming CAG/CTG repeats interfere with gap repair to cause repeat expansions and chromosome breaks.

Nature communications

Polleys EJ,Del Priore I,Haber JE,Freudenreich CH

PA5-34905 was used in ChIP Assay to develop an assay such that resection and ssDNA gap fill-in would occur across a (CAG)70 or (CTG)70 repeat tract.

Sat Apr 29 00:00:00 UTC 2023

Rad9/53BP1 promotes DNA repair via crossover recombination by limiting the Sgs1 and Mph1 helicases.

Nature communications

Ferrari M,Rawal CC,Lodovichi S,Vietri MY,Pellicioli A

PA5-34905 was used in Chromatin immunoprecipitation to shed light on how cells couple DNA damage checkpoint (DDC) with the choice and effectiveness of homologous recombination (HR) sub-pathways, with implications for genome instability and cancer.

Tue Jun 23 00:00:00 UTC 2020

Rad9/53BP1 promotes DNA repair via crossover recombination by limiting the Sgs1 and Mph1 helicases.

Nature communications

Ferrari M,Rawal CC,Lodovichi S,Vietri MY,Pellicioli A

Tue Jun 23 00:00:00 UTC 2020

Defects in the GINS complex increase the instability of repetitive sequences via a recombination-dependent mechanism.

PLoS genetics

Jedrychowska M,Denkiewicz-Kruk M,Alabrudzinska M,Skoneczna A,Jonczyk P,Dmowski M,Fijalkowska IJ

PA5-34905 was used in Western Blotting to propose that defective functioning of the CMG-E complex in psf1-1 cells impairs the progression of DNA replication what increases the contribution of repair mechanisms such as template switch and break-induced replication.

Sun Dec 01 00:00:00 UTC 2019

Lack of G1/S control destabilizes the yeast genome via replication stress-induced DSBs and illegitimate recombination.

Journal of cell science

Krol K,Antoniuk-Majchrzak J,Skoneczny M,Sienko M,Jendrysek J,Rumienczyk I,Halas A,Kurlandzka A,Skoneczna A

PA5-34905 was used in Western Blotting to show that a lack of Swi6 causes replication stress leading to double-strand break formation, inefficient DNA repair and DNA content alterations, resulting in high cell mortality.