Invitrogen

RAD51C Polyclonal Antibody

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RAD51C Antibody (PA5-95324) in WB

Western blot analysis of Rad51C in, Lane 1: human 293T whole cell lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. The membrane was blocked with 5% non-fat milk/TBS for 1. 5 hour at RT. The membrane was incubated with RAD51C Polyclonal Antibody (Product # PA5-95324) at 0.5 μg/... View More

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产品信息

PA5-95324

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence in the middle region of human Rad51C (177-207aa IQHLQLIAEKHKGEEHRKALEDFTLDNILSH).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2807127

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

ZFPM1 is a transcription regulator that plays an essential role in erythroid and megakaryocytic cell differentiation. ZFPM1 is an essential cofactor that acts via the formation of a heterodimer with transcription factors of the GATA family GATA1, GATA2 and GATA3. Such heterodimer can both activate or repress transcriptional activity, depending on the cell and promoter context. The heterodimer formed with GATA proteins is essential to activate expression of genes such as NFE2, ITGA2B, alpha- and beta-globin, while it represses expression of KLF1. ZFPM1 may be involved in regulation of some genes in gonads. ZFPM1 may also be involved in cardiac development, in a non-redundant way with ZFPM2/FOG2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。