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RAD52 Antibody (PA5-119963) in IHC (P)
Immunohistochemistry (Paraffin) analysis of paraffin-embedded human liver carcinoma tissue using RAD52 Polyclonal Antibody (Product # PA5-119963). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the RAD52 antibody at a di... View More
Product Details
PA5-119963
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide within human RAD52 aa 331-380/418
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2913535
Product Specific Information
Positive Control: Hela cell lysate, HL-60 cell lysate, human thyroid tissue, human colon carcinoma tissue, human liver carcinoma tissue, human spleen tissue, mouse liver tissue, rat spleen tissue.
Subcellular Location: Nucleus.
Target Information
DNA double-strand breaks can be generated by exposure to ionizing radiation or through endogenous cellular reactions. Double-strand breaks are generally thought to be repaired via one of two distinct mechanisms: homologous recombination (HR) or non-homologous end joining (NHEJ). Homologous recombination requires the activity of the members of the RAD52 epistasis group, which includeRAD50, RAD51, RAD52, RAD54, RDH54/TID1, RAD55, RAD57, RAD59, MRE11, and XRS2. RAD52 can catalyze strand annealing reactions, and can form oligomers that attach to ends of single-stranded DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA repair protein RAD52 homolog; recombination protein RAD52; rhabdomyosarcoma antigen MU-RMS-40.23
Gene Aliases: RAD52; Rad52yh
UniProt ID: (Human) P43351, (Mouse) P43352
Entrez Gene ID: (Human) 5893, (Rat) 297561, (Mouse) 19365
DNA recombinase assembly
double-strand break repair
DNA recombination
cellular response to DNA damage stimulus
DNA double-strand break processing involved in repair via single-strand annealing
protein sumoylation
cellular response to oxidative stress
regulation of nucleotide-excision repair
double-strand break repair via homologous recombination
DNA repair
double-strand break repair via single-strand annealing
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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