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RAG1 Antibody (PA5-88678) in WB
Western blot analysis of RAG1 in extracts of Mouse thymus. Samples were incubated with RAG1 Polyclonal antibody (Product # PA5-88678) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
PA5-88678
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human RAG1 (NP_0004391)
偶联物
形式
Liquid
浓度
0.33 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2805070
产品详细信息
Immunogen sequence: MAASFPPTLG LSSAPDEIQH PHIKFSEWKF KLFRVRSFEK TPEEAQKEKK DSFEGKPSLE QSPAVLDKAD GQKPVPTQPL LKAHPKFSKK FHDNEKARGK AIHQANLRHL CRICGNSFRA DEHNRRYPVH GPVDGKTLGL LRKKEKRATS WPDLIAKVFR IDVKADVDSI HPTEFCHNCW SIMHRKFSSA PCEVYFPRNV TMEWHPHTPS CDICNTARRG LKRKSLQPNL QLSKKLKTVL DQARQARQRK RRAQARISSK DVMKKIANCS; Positive Samples: Mouse thymus; Cellular Location: Nucleus
靶标信息
The protein encoded by this gene is involved in activation of immunoglobulin V-D-J recombination. The encoded protein is involved in recognition of the DNA substrate, but stable binding and cleavage activity also requires RAG2. Defects in this gene can be the cause of several diseases.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: MGC43321; RAG-1; RAG-1 Antibody; recombinase activating gene 1; recombination activating gene 1 Antibody; recombination activating protein 1; recombination-activating gene 1; RNF74 Antibody; V(D)J recombination-activating protein 1
基因别名: Rag-1; Rag1
UniProt ID: (Mouse) P15919
Entrez Gene ID: (Mouse) 19373
nucleic acid binding
DNA binding
catalytic activity
nuclease activity
endonuclease activity
ubiquitin-protein transferase activity
protein binding
zinc ion binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
hydrolase activity
hydrolase activity, acting on ester bonds
ligase activity
acid-amino acid ligase activity
ribosomal S6-glutamic acid ligase activity
histone binding
phosphoric ester hydrolase activity
protein homodimerization activity
sequence-specific DNA binding
T/G mismatch-specific endonuclease activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
retroviral 3' processing activity
metal ion binding
ubiquitin protein ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
DNA metabolism protein
endodeoxyribonuclease
nucleic acid metabolism protein
adaptive immune response
pre-B cell allelic exclusion
DNA recombination
metabolic process
histone monoubiquitination
obsolete death
chromatin modification
B cell differentiation
T cell differentiation in thymus
V(D)J recombination
T cell homeostasis
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
regulation of T cell differentiation
thymus development
protein autoubiquitination
negative regulation of T cell apoptotic process
negative regulation of thymocyte apoptotic process
nucleic acid phosphodiester bond hydrolysis
