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RALGAPA2 Antibody (PA5-101287) in ICC/IF
Immunocytochemistry analysis of RALGAPA2 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with RALGAPA2 polyclonal antibody (Product # PA5-101287) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-101287
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human RALGAPA2(Accession Q2PPJ7), corresponding to amino acid residues S646-S696.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2850726
Product Specific Information
Antibody detects endogenous levels of total AS250.
Target Information
Catalytic subunit of the heterodimeric RalGAP2 complex which acts as a GTPase activator for the Ras-like small GTPases RALA and RALB.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 250 kDa substrate of Akt; akt substrate AS250; AS250; P220; Ral GTPase activating protein, alpha subunit 2 (catalytic); ral GTPase-activating protein alpha subunit 2; Ral GTPase-activating protein subunit alpha-2; RapGAPalpha2
Gene Aliases: A230067G21Rik; AS250; bA287B20.1; BC053994; C20orf74; dJ1049G11; dJ1049G11.4; KIAA1272; p220; pp250; RALGAPA2; RGC2
UniProt ID: (Human) Q2PPJ7, (Mouse) A3KGS3
Entrez Gene ID: (Human) 57186, (Mouse) 241694
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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