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RALGAPA2 Antibody (PA5-101287) in ICC/IF
Immunocytochemistry analysis of RALGAPA2 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with RALGAPA2 polyclonal antibody (Product # PA5-101287) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-101287
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human RALGAPA2(Accession Q2PPJ7), corresponding to amino acid residues S646-S696.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2850726
产品详细信息
Antibody detects endogenous levels of total AS250.
靶标信息
Catalytic subunit of the heterodimeric RalGAP2 complex which acts as a GTPase activator for the Ras-like small GTPases RALA and RALB.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 250 kDa substrate of Akt; akt substrate AS250; AS250; P220; Ral GTPase activating protein, alpha subunit 2 (catalytic); ral GTPase-activating protein alpha subunit 2; Ral GTPase-activating protein subunit alpha-2; RapGAPalpha2
基因别名: A230067G21Rik; AS250; bA287B20.1; BC053994; C20orf74; dJ1049G11; dJ1049G11.4; KIAA1272; p220; pp250; RALGAPA2; RGC2
UniProt ID: (Human) Q2PPJ7, (Mouse) A3KGS3
Entrez Gene ID: (Human) 57186, (Mouse) 241694
